The structural characterization of the O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli serotype O118 and its relation to the O-antigens of Escherichia coli O151 and Salmonella enterica O47

被引：4

作者：

MacLean, Leann L. ^{[1
]}

Liu, Yanhong ^{[2
]}

Vinogradov, Evgeny ^{[1
]}

Perry, Malcolm B. ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

[2] Eastern Reg Ctr, Agr Res Serv, Dept Agr, Wyndmoor, PA 19038 USA

来源：

CARBOHYDRATE RESEARCH | 2010年 / 345卷 / 18期

关键词：

Escherichia coli; O Antigen; Structure; Polysaccharide; NMR; ABSOLUTE-CONFIGURATION; CITROBACTER-FREUNDII; GENE-CLUSTER; MONOSACCHARIDES; CHAIN;

D O I：

10.1016/j.carres.2010.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mild acid hydrolysis of the lipopolysaccharide produced by Escherichia coli 0118 H16 standard strain (NRCC 6613) afforded an O-polysaccharide (O-PS) composed of a-galactose 2-acetamidoylamino-2 6-dideoxy-L-galactose 2-acetamido-2-deoxy-D-glucose ribitol and phosphate (1 1 1 1 1) From DOC-PAGE sugar and methylation analyses one- and two-dimensional NMR spectroscopy capillary electrophoresis-mass spectrometry hydrolysis and sequential Smith-type periodate oxidation studies the O-PS was determined to be an unbranched linear polymer having the structure [6)-alpha-D-Galp-(1 -> 3)-alpha-L-FucpNAm-(1 -> 3)-beta-D-GlcpNAc-(1 -> 3)-Rib-ol-5-P-(0 ->](n) The structure of the O-PS is consistent with the reported DNA data on the O-antigen gene-cluster of E coli 0118 and interestingly the O-PS is similar to the structures of the O-antigens of Salmonella enterica 047 and E colt 0151 H10 reference strain 880-67 as predicted from the results of DNA sequencing of their respective O-antigen gene-clusters (C) 2010 Elsevier Ltd All rights reserved

引用

页码：2664 / 2669

页数：6

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