Nuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cells

被引：44

作者：

Duarte, Iola F. ^{[1
]}

Lamego, Ines ^{[2
]}

Marques, Joana ^{[1
]}

Marques, M. Paula M. ^{[2
]}

Blaise, Benjamin J. ^{[3
]}

Gil, Ana M. ^{[1
]}

机构：

[1] Univ Aveiro, CICECO Dept Quim, P-3810193 Aveiro, Portugal

[2] Univ Coimbra, Fac Sci & Technol, Dept Life Sci, R&D Unit Mol Phys Chem, P-3000 Coimbra, Portugal

[3] Univ Lyon, Ctr RMN Tres Hauts Champs, F-69100 Villeurbanne, France

来源：

JOURNAL OF PROTEOME RESEARCH | 2010年 / 9卷 / 11期

关键词：

osteosarcoma; MG-63; cells; cisplatin; cancer; cancer therapy; metabonomics; NMR spectroscopy; DOSE; 2; GY; BREAST-CANCER; 3-DIMENSIONAL SPHEROIDS; IONIZING-RADIATION; MOBILE LIPIDS; K562; CELLS; IN-VITRO; H-1-NMR; SPECTROSCOPY; APOPTOSIS;

D O I：

10.1021/pr100635n

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, H-1 HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.

引用

页码：5877 / 5886

页数：10

共 33 条

[1] Noninvasive magnetic resonance spectroscopic pharmacodynamic markers of the choline kinase inhibitor MN58b in human carcinoma models [J].

Al-Saffar, NMS ;

Troy, H ;

Ramírez de Molina, A ;

Jackson, LE ;

Madhu, B ;

Griffiths, JR ;

Leach, MO ;

Workman, P ;

Lacal, JC ;

Judson, IR ;

Chung, YL .

CANCER RESEARCH, 2006, 66 (01) :427-434

[2] Statistical Recoupling Prior to Significance Testing in Nuclear Magnetic Resonance Based Metabonomics [J].

Blaise, Benjamin J. ;

Shintu, Laetitia ;

Elena, Benedicte ;

Emsley, Lyndon ;

Dumas, Marc-Emmanuel ;

Toulhoat, Pierre .

ANALYTICAL CHEMISTRY, 2009, 81 (15) :6242-6251

[3] N-(4-iodophenyl)-N′-(2-chloroethyl) urea as a microtubule disrupter:: in vitro and in vivo profiling of antitumoral activity on CT-26 murine colon carcinoma cell line cultured and grafted to mice [J].

Borel, M. ;

Degoul, F. ;

Communal, Y. ;

Mounetou, E. ;

Bouchon, B. ;

C-Gaudreault, R. ;

Madelmont, J. C. ;

Miot-Noirault, E. .

BRITISH JOURNAL OF CANCER, 2007, 96 (11) :1684-1691

[4] Two-step formation of lH NMR visible mobile lipids during apoptosis of paclitaxel-treated K562 cells [J].

Brisdelli, F ;

Iorio, E ;

Knijn, A ;

Ferretti, A ;

Marcheggiani, D ;

Lenti, L ;

Strom, R ;

Podo, F ;

Bozzi, A .

BIOCHEMICAL PHARMACOLOGY, 2003, 65 (08) :1271-1280

[5] Chemotherapy resistance in osteosarcoma: current challenges and future directions [J].

Chou, Alexander J. ;

Gorlick, Richard .

EXPERT REVIEW OF ANTICANCER THERAPY, 2006, 6 (07) :1075-1085

[6] Therapy for osteosarcoma: Where do we go from here? [J].

Chou A.J. ;

Geller D.S. ;

Gorlick R. .

Pediatric Drugs, 2008, 10 (5) :315-327

[7]

CHUNG YL, 2008, USING METABONOMICS M, P55

[8] 1H NMR visible lipids are induced by phosphonium salts and 5-fluorouracil in human breast cancer cells [J].

Cooper, WA ;

Bartier, WA ;

Rideout, DC ;

Delikatny, EJ .

MAGNETIC RESONANCE IN MEDICINE, 2001, 45 (06) :1001-1010

[9] NMR metabonomics for mammalian cell metabolism studies [J].

Duarte, Iola F. ;

Lamego, Ines ;

Rocha, Claudia ;

Gil, Ana M. .

BIOANALYSIS, 2009, 1 (09) :1597-1614

[10] Analytical Approaches toward Successful Human Cell Metabolome Studies by NMR Spectroscopy [J].

Duarte, Iola F. ;

Marques, Joana ;

Ladeirinha, Ana F. ;

Rocha, Claudia ;

Lamego, Ines ;

Calheiros, Rita ;

Silva, Tania M. ;

Marques, M. Paula M. ;

Melo, Joana B. ;

Carreira, Isabel M. ;

Gil, Ana M. .

ANALYTICAL CHEMISTRY, 2009, 81 (12) :5023-5032

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