Expression and production of bioactive human interleukin-18 in transgenic tobacco plants

被引：23
作者：
Zhang, B
Yang, YH
Lin, YM
Rao, Q
Zheng, GG
Wu, KF [1 ]
机构：
[1] Chinese Acad Med Sci, Inst Hematol, Natl Lab Expt Hematol, Tianjin, Peoples R China
[2] Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
[3] Peking Union Med Coll, Tianjin, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 19期
关键词：
bioactivity; human IL-18; interleukin-18; tobacco; transgenic plants;
D O I：
10.1023/A:1025638623770
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-gamma on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g(-1) tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.
引用
收藏
页码：1629 / 1635
页数：7
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