Comparative study of hyaluronic derivatives: rheological behaviour, mechanical and chemical degradation

被引：24

作者：

Prieto, JG ^{[1
]}

Pulido, MM ^{[1
]}

Zapico, J ^{[1
]}

Molina, AJ ^{[1
]}

Gimeno, M ^{[1
]}

Coronel, P ^{[1
]}

Alvarez, AI ^{[1
]}

机构：

[1] Univ Leon, Dept Physiol, Leon 24071, Spain

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2005年 / 35卷 / 1-2期

关键词：

sodium hyaluronate solutions; hylans; viscosity;

D O I：

10.1016/j.ijbiomac.2004.12.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Depolymerisation by oxytetracycline (OTC) as well as the progressive cleavage of hyaluronic acid induced by ultrasound was investigated in nine commercially available hyaluronic polymers. Sample solutions differed in molecular weight, from 500 to 7000 kDa, and in their source. The hyaluronic acid concentration in each sample was analysed by HPLC. The concentration range was over 8.39-10.18 mg ml(-1) in samples with a nominal concentration of 1%, and 14.05 mg ml(-1) in one sample with a nominal concentration of 1.5%. It was found that stability was dependent on both molecular weight and the concentration of the samples. The rheological parameters n (power law index) and K (consistency coefficient) were good predictors regarding the degradation behaviour. Although many factors are involved in obtaining a therapeutic response, the results obtained in this work support the notion that both mechanical and chemical degradation are reduced in hyaluronate solutions with low molecular weight, the final concentration of the product being a critical factor. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：63 / 69

页数：7

共 32 条

[1] A risk-benefit assessment of injections of hyaluronan and its derivatives in the treatment of osteoarthritis of the knee [J].