Ultrafast Yb:KGd(WO4)2 laser for multimodal biomedical imaging with reduced photodamage

被引：31

作者：

Major, Arkady ^{[1
]}

Cisek, Richard ^{[1
]}

Tuer, Adam ^{[1
]}

Prent, Nicole ^{[1
]}

Barzda, Virginijus ^{[1
]}

机构：

[1] Univ Manitoba, Dept Elect & Comp Engn, 75 Chancellors Circle, Winnipeg, MB R3T 5V6, Canada

来源：

COMMERCIAL AND BIOMEDICAL APPLICATIONS OF ULTRAFAST LASERS VIII | 2008年 / 6881卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

femtosecond lasers; diode-pumped solid-state lasers; nonlinear microscopy; biomedical imaging;

D O I：

10.1117/12.763953

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report on the development and successful application of a femtosecond Yb:KGd(WO4)(2) laser for multimodal imaging of various biological samples. Its operation at longer wavelengths, 1029 nm, provided efficient excitation and greatly reduced sample photobleaching. The laser produced similar to 300 fs pulses with up to 100 nJ of energy at 14.3 MHz repetition rate. This laser system enabled continuous imaging of various live samples for prolonged periods of time. The details of laser development and fluorescence imaging of isolated chloroplasts are reported.

引用

页数：7

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