Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

被引：14
作者：
Misonou, H
Nishiki, T
Sekiguchi, M
Takahashi, M
Kamata, Y
Kozaki, S
OharaImaizumi, M
Kumakura, K
机构：
[1] SOPHIA UNIV,INST LIFE SCI,CHIYODA KU,TOKYO 102,JAPAN
[2] MITSUBISHI KASEI INST LIFE SCI,MACHIDA,TOKYO 194,JAPAN
[3] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET SCI,SAKAI,OSAKA 593,JAPAN
来源：
BRAIN RESEARCH | 1996年 / 737卷 / 1-2期
关键词：
exocytosis; SNAP-25; VAMP-2; Botulinum neurotoxin; MgATP;
D O I：
10.1016/0006-8993(96)00954-7
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
in digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.
引用
收藏
页码：351 / 355
页数：5
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