Synergistic effect of tetrandrine and ethidium bromide against methicillin-resistant Staphylococcus aureus (MRSA)

被引:1
作者
Lee, Young-Seob [3 ]
Han, Sin-Hee [3 ]
Lee, Su-Hwan [3 ]
Kim, Young-Guk [3 ]
Park, Chung-Berm [3 ]
Kang, Ok-Hwa [1 ,2 ]
Keum, Joon-Ho [1 ,2 ]
Kim, Sung-Bae [1 ,2 ]
Mun, Su-Hyun [1 ,2 ]
Shin, Dong-Won [4 ]
Kwon, Dong-Yeul [1 ,2 ]
机构
[1] Wonkang Univ, Coll Pharm, Iksan 570749, Chonbuk, South Korea
[2] Wonkang Univ, Wonkwang Oriental Med Res Inst, Iksan 570749, Chonbuk, South Korea
[3] Natl Inst Hort & Herbal Sci, RDA, Dept Herbal Crop Res, Eumsung 369873, Chonbuk, South Korea
[4] Sunchon Natl Univ, Dept Oriental Med Resources, Sunchon 540742, Jeonnam, South Korea
关键词
Staphylococcus aureus; Ethidium bromide; Tetrandrine; Antibacterial activity; GRAM-NEGATIVE BACTERIA; EFFLUX PUMPS; COMBINATION; ANTIBACTERIAL; INHIBITOR; LACTAMS; GALLATE;
D O I
暂无
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Methicillin-resistant Staphylococcus aureus (MRSA) along with other resistant bacteria have become a significant social and clinical problem. Therefore, there is an urgent need to develop bioactive compounds from natural products as alternatives to the very few antibiotics that remain effective. Recently, the efflux mechanism has been identified as the main contributor to antibiotic resistance in bacteria. This study therefore aimed to evaluate tetrandrine (TET), an efflux pump inhibitor (EPI), as a potential antibiotic against MRSA. We investigated the antimicrobial activity of TET against 17 MRSA strains, of which 3 selected strains were studied in further detail using a time-kill assay. When these bacterial strains (1 x 10(6) colony-forming units (cfu)/ml) were incubated with TET in a time-kill assay, log-scale bactericidal activity was observed, which lasted for 24 hr. In addition, TET exhibits a synergistic effect when combined with the multi-drug resistance (MDR)-efflux pump substrate ethidium bromide (EtBr). Structure-function studies of the antibiotic activity of TET in combination with EtBr may lead to the discovery of more effective efflux pump inhibitors.
引用
收藏
页码:645 / 651
页数:7
相关论文
共 29 条
[1]   Antibacterial Activity of Ecklonia cava Against Methicillin-Resistant Staphylococcus aureus and Salmonella spp. [J].
Choi, Jang-Gi ;
Kang, Ok-Hwa ;
Brice, Obiang-Obounou ;
Lee, Young-Seob ;
Chae, Hee-Sung ;
Oh, You-Chang ;
Sohn, Dong-Hwan ;
Park, Hyun ;
Choi, Han-Gil ;
Kim, Se-Gun ;
Shin, Dong-Won ;
Kwon, Dong-Yeul .
FOODBORNE PATHOGENS AND DISEASE, 2010, 7 (04) :435-441
[2]   In Vitro Activity of Methyl Gallate Isolated from Galla Rhois Alone and in Combination with Ciprofloxacin Against Clinical Isolates of Salmonella [J].
Choi, Jang-Gi ;
Kang, Ok-Hwa ;
Lee, Young-Seob ;
Oh, You-Chang ;
Chae, Hee-Sung ;
Jang, Hye-Jin ;
Kim, Jong Hak ;
Sohn, Dong-Hwan ;
Shin, Dong-Won ;
Park, Hyun ;
Kwon, Dong-Yeul .
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 18 (11) :1848-1852
[3]   Efflux-related resistance to norfloxacin, dyes, and biocides in bloodstream isolates of Staphylococcus aureus [J].
DeMarco, Carmen E. ;
Cushing, Laurel A. ;
Frempong-Manso, Emmanuel ;
Seo, Susan M. ;
Jaravaza, Tinevimbo A. A. ;
Kaatz, Glenn W. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (09) :3235-3239
[4]  
FANG DC, 1986, CHINESE MED J-PEKING, V99, P638
[5]   Antimicrobial activity of clove and rosemary essential oils alone and in combination [J].
Fu, Yujie ;
Zu, YuanGang ;
Chen, Liyan ;
Shi, XiaoGuang ;
Wang, Zhe ;
Sun, Su ;
Efferth, Thomas .
PHYTOTHERAPY RESEARCH, 2007, 21 (10) :989-994
[6]   A novel inhibitor of multidrug efflux pumps in Staphylococcus aureus [J].
Gibbons, S ;
Oluwatuyi, M ;
Kaatz, GW .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 51 (01) :13-17
[7]   Anti-staphylococcal plant natural products [J].
Gibbons, S .
NATURAL PRODUCT REPORTS, 2004, 21 (02) :263-277
[8]   Tetrandrine [J].
Huang, YT ;
Hong, CY .
CARDIOVASCULAR DRUG REVIEWS, 1998, 16 (01) :1-15
[9]   Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (Alleles), and human disease [J].
Jarraud, S ;
Mougel, C ;
Thioulouse, J ;
Lina, G ;
Meugnier, H ;
Forey, F ;
Nesme, X ;
Etienne, J ;
Vandenesch, F .
INFECTION AND IMMUNITY, 2002, 70 (02) :631-641
[10]   Synergistic antibacterial effect between silybin and N,N'-dicyclohexylcarbodiimide in clinical Pseudomonas aeruginosa isolates [J].
Jung, Hyun Jun ;
Lee, Dong Gun .
JOURNAL OF MICROBIOLOGY, 2008, 46 (04) :462-467