Real-time Fluorescence and Visual Colorimetric Loop-Mediated Isothermal Amplification Assays for the Rapid and Visual Identification of the Genus Diodon

被引：7
作者：
Xie, Ruibin [1 ]
Gao, Jie [1 ]
Li, Hui [1 ]
Yu, Wenjie [1 ]
Zhang, Juan [1 ]
Wang, Nan [1 ]
Chen, Ailiang [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Safety, Beijing 100081, Peoples R China
来源：
FOOD ANALYTICAL METHODS | 2022年 / 15卷 / 09期
关键词：
Diodon; Loop-mediated isothermal amplification; Pufferfish species identification; Food safety; Visual detection; Fluorescence detection; SPECIES IDENTIFICATION; FISH FILLET; TETRODOTOXIN; PISCES; FOOD; DNA;
D O I：
10.1007/s12161-022-02307-1
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In the present study, real-time fluorescence loop-mediated isothermal amplification (flo-LAMP) and visual colorimetric (vis-LAMP) assays were established for Diodon identification. LAMP primers were designed for the Diodon cytochrome oxidase I gene, and their high specificity was validated against 21 other fish species. LAMP has a minimum detection limit of 0.5 pg/mu L for genomic DNA. Furthermore, LAMP identified Diodon in both cooked and digested samples, with the results showing high application potential. The set of LAMP assays was carried out within 1 h under constant temperature conditions. For the flo-LAMP, a positive result was judged by an S-shaped amplification curve. For the vis-LAMP, the results were observed with the naked eye during daylight. Therefore, these rapid, reliable, sensitive, and inexpensive Diodon identification methods could be used to monitor the presence of fish from the Diodon genus in the food industry and detect cases of Diodon poisoning.
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收藏
页码：2487 / 2495
页数：9
相关论文
共 35 条
[1]   Morphological identification and molecular validation of anchovies (Engraulidae) in the Persian Gulf and Oman Sea [J].
Afrand, Mahboobeh ;
Sourinejad, Iman ;
Fazeli, Seyed Abolhassan Shahzadeh ;
Akbarzadeh, Arash ;
Yeganeh, Laleh Parsa ;
Sadeghi, Maryam ;
Azarbaijani, Reza .
ZOOTAXA, 2020, 4742 (02) :375-391
[2]   Neogene Proto-Caribbean porcupinefishes (Diodontidae) [J].
Aguilera, Orange ;
Andrade Silva, Guilherme Oliveira ;
Lopes, Ricardo Tadeu ;
Machado, Alessandra Silveira ;
dos Santos, Thais Maria ;
Marques, Gabriela ;
Bertucci, Thayse ;
Aguiar, Thayanne ;
Carrillo-Briceno, Jorge ;
Rodriguez, Felix ;
Jaramillo, Carlos .
PLOS ONE, 2017, 12 (07)
[3]   PCR-RFLP Detection and Genogroup Identification of Piscirickettsia salmonis in Field Samples [J].
Aravena, Pamela ;
Pulgar, Rodrigo ;
Ortiz-Severin, Javiera ;
Maza, Felipe ;
Gaete, Alexis ;
Martinez, Sebastian ;
Seron, Ervin ;
Gonzalez, Mauricio ;
Cambiazo, Veronica .
PATHOGENS, 2020, 9 (05)
[4]   Integrity of nuclear genomic deoxyribonucleic acid in cooked meat: Implications for food traceability [J].
Aslan, Oe ;
Hamill, R. M. ;
Sweeney, T. ;
Reardon, W. ;
Mullen, A. M. .
JOURNAL OF ANIMAL SCIENCE, 2009, 87 (01) :57-61
[5]   Comparative study of different methods for the extraction of DNA from fish species commercially available in South Africa [J].
Cawthorn, Donna-Maree ;
Steinman, Harris Andrew ;
Witthuhn, R. Corli .
FOOD CONTROL, 2011, 22 (02) :231-244
[6]   Differentiation of fish species in Taiwan Strait by PCR-RFLP and lab-on-a-chip system [J].
Chen, Shuangya ;
Zhang, Yongxiang ;
Li, Hong ;
Wang, Jiahe ;
Chen, Weiling ;
Zhou, Yu ;
Zhou, Shan .
FOOD CONTROL, 2014, 44 :26-34
[7]   A Rapid, Simple, Inexpensive, and Mobile Colorimetric Assay COVID-19-LAMP for Mass On-Site Screening of COVID-19 [J].
Chow, Franklin Wang-Ngai ;
Chan, Tony Tat-Yin ;
Tam, Anthony Raymond ;
Zhao, Suhui ;
Yao, Weiming ;
Fung, Joshua ;
Cheng, Flora Ka-Kei ;
Lo, George Chi-Shing ;
Chu, Stella ;
Aw-Yong, Kam Leng ;
Tang, James Yat-Man ;
Tsang, Chi-Ching ;
Luk, Hayes Kam-Hei ;
Wong, Antonio Cheuk-Pui ;
Li, Kenneth Sze-Ming ;
Zhu, Longchao ;
He, Zirong ;
Tam, Emily Wan Ting ;
Chung, Tom Wai-Hin ;
Wong, Sally Cheuk Ying ;
Que, Tak-Lun ;
Fung, Kitty Sau-Chun ;
Lung, David Christopher ;
Wu, Alan Ka-Lun ;
Hung, Ivan Fan-Ngai ;
Woo, Patrick Chiu-Yat ;
Lau, Susanna Kar-Pui .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (15) :1-10
[8]  
CHUANJIANG Z, 2019, J HENAN NORMAL U NAT, V47, P7
[9]   Species identification and quantification in meat and meat products using droplet digital PCR (ddPCR) [J].
Floren, C. ;
Wiedemann, I. ;
Brenig, B. ;
Schuetz, E. ;
Beck, J. .
FOOD CHEMISTRY, 2015, 173 :1054-1058
[10]   Severe seafood poisoning in French Polynesia: A retrospective analysis of 129 medical files [J].
Gatti, C. ;
Oelher, E. ;
Legrand, A. M. .
TOXICON, 2008, 51 (05) :746-753
1234