The effects of Levothyroxine on the structure and dynamics of DPPC liposome: FTIR and DSC studies

被引:25
作者
Aleskndrany, Abeer [1 ]
Sahin, Ipek [1 ]
机构
[1] Ege Univ, Fac Sci, Dept Phys, TR-35100 Izmir, Turkey
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2020年 / 1862卷 / 06期
关键词
Levothyroxine; Dipalmitoyl phosphatidylcholine; Drug-membrane interaction; Fourier transform infrared; Differential scanning calorimetry; TRANSFORM INFRARED-SPECTROSCOPY; PHOSPHOLIPID MODEL MEMBRANES; L-TRIIODOTHYRONINE BINDING; THYROID-HORMONES; LIPID-PEROXIDATION; OXIDATIVE STRESS; PHASE-TRANSITIONS; MOLECULAR ORDER; GEL PHASE; THYROXINE;
D O I
10.1016/j.bbamem.2020.183245
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Levothyroxine (3,5,3',5'-tetraiodothyronine), which is a L-isomer of thyroxine T4 (L-T4), is a synthetic thyroid hormone that is biochemically and physiologically indistinguishable from endogenous T4. It is used as a thyroid hormone replacement drug to treat an underactive thyroid gland. The interaction of L-T4, with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) was studied in the presence (1 mol%, 3 mol%, 6 mol%, 9 mol%, 15 mol%, 24 mol% and 30 mol%) and absence of L-T4 by using two different non-invasive techniques; Fourier Transform Infrared (FTIR) spectroscopy and Differential Scanning Calorimetry (DSC). The results show that L-T4 does perturb the phase transition profile by either decreasing the main transition temperature (T-m) and enthalpy (Delta H) or increasing the width at half height (Delta T-1/2). That means; it changes the physical properties of DPPC bilayers. Addition of L-T4 into pure DPPC liposomes shifts the phase transition to lower temperature, disorder the system in gel phase with opposite effect in liquid-crystalline phase and increases the dynamics of the system in both phases and also causes dehydration of the groups of lipids and the water molecules around.
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页数:10
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