Production of recombinant serpins in Escherichia coli

被引：21
|
作者：
Bird, PI [1 ]
Pak, SC
Worrall, DM
Bottomley, SP
机构：
[1] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
[2] Harvard Univ, Childrens Hosp, Sch Med, Dept Pediat, Boston, MA 02115 USA
[3] Natl Univ Ireland Univ Coll Dublin, Dept Biochem, Dublin 4, Ireland
[4] Natl Univ Ireland Univ Coll Dublin, Conway Inst, Dublin 4, Ireland
来源：
METHODS | 2004年 / 32卷 / 02期
基金：
英国医学研究理事会; 澳大利亚研究理事会;
关键词：
serpin; recombinant protein; expression systems; Escherichia coli;
D O I：
10.1016/S1046-2023(03)00208-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Expression. systems based on Escherichia coli offer fast, cheap, and convenient means for the production of recombinant serpins. Over 30 active serpins from prokaryotic and eukaryotic organisms have been produced in this way, using a variety of vectors, promoters, fusion partners, and host strains. Serpins forming insoluble inclusion bodies in E coli can generally be solubilized and refolded. Here, we outline the general approaches and procedures to be considered when contemplating the use of E coli for recombinant serpin production. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码：169 / 176
页数：8
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