Characterization of an oil-degrading Microcoleus consortium by means of confocal scanning microscopy, scanning electron microscopy and transmission electron microscopy

被引：0

作者：

Diestra, E ^{[1
]}

Solé, A

Martí, M

de Oteyza, TG

Grimalt, JO

Esteve, I

机构：

[1] Autonomous Univ Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

[2] Autonomous Univ Barcelona, Microscopy Serv, Bellaterra 08193, Spain

[3] CSIC, IIQAB, Dept Environm Chem, Barcelona, Spain

来源：

SCANNING | 2005年 / 27卷 / 04期

关键词：

Microcoleus consortium; confocal laser scanning microscopy; transmission electron microscopy; scanning electron microscopy; crude oil;

D O I：

暂无

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A consortium of microorganisms with the capacity to degrade crude oil has been characterized by means of confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The analysis using CLSM shows that Microcoleus chthonoplastes is the dominant organism in the consortium. This cyanobacterium forms long filaments that group together in bundles inside a mucopolysaccharide sheath. Scanning electron microscopy and transmission electron microscopy have allowed us to demonstrate that this cyanobacterium forms a consortium primarily with three morphotypes of the heterotrophic microorganisms found in the Microcoleus chthonoplastes sheath. The optimal growth of Microcoleus consortium was obtained in presence of light and crude oil, and under anaerobic conditions. When grown in agar plate, only one type of colony (green and filamentous) was observed.

引用

页码：176 / 180

页数：5

共 23 条

[1] Evidence for n-alkane consumption and oxidation by filamentous cyanobacteria from oil-contaminated coasts of the Arabian Gulf [J].

Al-Hasan, RH ;

Al-Bader, DA ;

Sorkhoh, NA ;

Radwan, SS .

MARINE BIOLOGY, 1998, 130 (03) :521-527

[2] UTILIZATION OF HYDROCARBONS BY CYANOBACTERIA FROM MICROBIAL MATS ON OILY COASTS OF THE GULF [J].

ALHASAN, RH ;

SORKHOH, NA ;

ALBADER, D ;

RADWAN, SS .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1994, 41 (05) :615-619

[3]

Castenholz RW., 2001, Bergey's Manual of Systematic Bacteriology ed, P473, DOI DOI 10.1007/978-0-387-21609-6_27

[4] Changes in the composition of polar and apolar crude oil fractions under the action of Microcoleus consortia [J].

De Oteyza, TG ;

Grimalt, JO ;

Diestra, E ;

Solé, A ;

Esteve, I .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 66 (02) :226-232

[5] A comparative study of cyanobacterial diversity in polluted and unpolluted microbial mats by means CLSM [J].

Diestra, E ;

Solé, A ;

Esteve, I .

OPHELIA, 2004, 58 (03) :151-156

[6] A QUANTITATIVE ULTRASTRUCTURAL-STUDY OF CHROMATIUM-MINUS IN THE BACTERIAL LAYER OF LAKE CISO (SPAIN) [J].

ESTEVE, I ;

MONTESINOS, E ;

MITCHELL, JG ;

GUERRERO, R .

ARCHIVES OF MICROBIOLOGY, 1990, 153 (04) :316-323

[7]

ESTEVE I, 1994, NATO ASI SERIES G, V35, P4165

[8] Characterization of functional bacterial groups in a hypersaline microbial mat community (Salins-de-Giraud, Camargue, France) [J].

Fourans, A ;

de Oteyza, TG ;

Wieland, A ;

Solé, A ;

Diestra, E ;

van Bleijswijk, J ;

Grimalt, JO ;

Kühl, M ;

Esteve, I ;

Muyzer, G ;

Caumette, P ;

Duran, R .

FEMS MICROBIOLOGY ECOLOGY, 2004, 51 (01) :55-70

[9] New polynuclear 4,4′-bis-1,2,4-triazole Fe(II) spin crossover compounds [J].

Garcia, Y ;

Ksenofontov, V ;

Gütlich, P .

COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II FASCICULE C-CHIMIE, 2001, 4 (03) :227-233

[10]

LLIROS M, 2003, FORMATEX MICROSCOPY, V1, P483

← 1 2 3 →