Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

被引：8

作者：

Kitagawa, Masae ^{[1
]}

Nagamine, Kentaro ^{[2
]}

Oka, Hiroko ^{[1
,3
]}

Ouhara, Kazuhisa ^{[4
]}

Ogawa, Ikuko ^{[1
]}

Komatsuzawa, Hitoshi ^{[4
]}

Kurihara, Hidemi ^{[1
,5
]}

机构：

[1] Hiroshima Univ Hosp, Ctr Oral Clin Examinat, Hiroshima, Japan

[2] Hiroshima Int Univ, Fac Hlth Sci, Hiroshima, Japan

[3] Hiroshima Univ, Ctr Cause Death Invest Res & Educ, Grad Sch Biomed & Hlth Sci, Hiroshima, Japan

[4] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Bacteriol, Hiroshima, Japan

[5] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Periodontal Med, Hiroshima, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2020年 / 605卷

基金：

日本学术振兴会;

关键词：

cnm gene; Streptococcus mtaans; Loop-mediated isothermal amplification; DIAGNOSIS; ASSAY; LAMP;

D O I：

10.1016/j.ab.2020.113812

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnmpositive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.

引用

收藏

页数：3

相关论文

共 20 条

[1] Operational feasibility of using loop-mediated isothermal amplification for diagnosis of pulmonary tuberculosis in microscopy centers of developing countries [J].

Boehme, Catharina C. ;

Nabeta, Pamela ;

Henostroza, German ;

Raqib, Rubhana ;

Rahim, Zeaur ;

Gerhardt, Martina ;

Sanga, Erica ;

Hoelscher, Michael ;

Notomi, Tsugunori ;

Hase, Tetsu ;

Perkins, Mark D. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) :1936-1940

[2] The Relationship of the Oral Microbiotia to Periodontal Health and Disease [J].

Curtis, Michael A. ;

Zenobia, Camille ;

Darveau, Richard P. .

CELL HOST & MICROBE, 2011, 10 (04) :302-306

[3] Detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification (LAMP) assay and serology in pediatric community-acquired pneumonia [J].

Gotoh, Kensei ;

Nishimura, Naoko ;

Ohshima, Yasunori ;

Arakawa, Yasuko ;

Hosono, Haruki ;

Yamamoto, Yasuto ;

Iwata, Yasushi ;

Nakane, Kazumasa ;

Funahashi, Keiji ;

Ozaki, Takao .

JOURNAL OF INFECTION AND CHEMOTHERAPY, 2012, 18 (05) :662-667

[4] An integrated direct loop-mediated isothermal amplification microdevice incorporated with an immunochromatographic strip for bacteria detection in human whole blood and milk without a sample preparation step [J].

Lee, Dohwan ;

Kim, Yong Tae ;

Lee, Jee Won ;

Kim, Do Hyun ;

Seo, Tae Seok .

BIOSENSORS & BIOELECTRONICS, 2016, 79 :273-279

[5]

Mansour TR, 2017, CUREUS J MED SCIENCE, V9, DOI 10.7759/cureus.1264

[6] Relationship between Cnm-positive Streptococcus mutans and cerebral microbleeds in humans [J].

Miyatani, F. ;

Kuriyama, N. ;

Watanabe, I. ;

Nomura, R. ;

Nakano, K. ;

Matsui, D. ;

Ozaki, E. ;

Koyama, T. ;

Nishigaki, M. ;

Yamamoto, T. ;

Mizuno, T. ;

Tamura, A. ;

Akazawa, K. ;

Takada, A. ;

Takeda, K. ;

Yamada, K. ;

Nakagawa, M. ;

Ihara, M. ;

Kanamura, N. ;

Friedland, R. P. ;

Watanabe, Y. .

ORAL DISEASES, 2015, 21 (07) :886-893

[7] A novel and rapid loop-mediated isothermal amplification assay for the specific detection of Verticillium dahliae [J].

Moradi, A. ;

Almasi, M. A. ;

Jafary, H. ;

Mercado-Blanco, J. .

JOURNAL OF APPLIED MICROBIOLOGY, 2014, 116 (04) :942-954

[8] Isolation of single-stranded DNA from loop-mediated isothermal amplification products [J].

Nagamine, K ;

Kuzuhara, Y ;

Notomi, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 290 (04) :1195-1198

[9] Accelerated reaction by loop-mediated isothermal amplification using loop primers [J].

Nagamine, K ;

Hase, T ;

Notomi, T .

MOLECULAR AND CELLULAR PROBES, 2002, 16 (03) :223-229

[10]

Nagamine K, 2001, CLIN CHEM, V47, P1742