Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro

被引:26
作者
Pan, Xiao-Ben [1 ]
Ma, Hui [1 ]
Jin, Qian [1 ]
Wei, Lai [1 ]
机构
[1] Peking Univ, Peking Univ Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
基金
中国国家自然科学基金;
关键词
hepatitis B virus; HepG2; interferon; microRNA; peripheral blood mononuclear cell; EPSTEIN-BARR-VIRUS; X PROTEIN; INTERFERON-ALPHA; HEPATOCELLULAR-CARCINOMA; VIRAL MICRORNAS; CELLS; INHIBITION; INFECTION; THERAPY; TRANSCRIPTION;
D O I
10.1111/j.1440-1746.2011.06979.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background and Aim: Alpha interferon (IFN-alpha) is an approved treatment for chronic hepatitis B (CHB). MicroRNA (miRNA) are currently known as a part of IFN-mediated antiviral defense. We aimed at characterizing the miRNA expression associated with hepatitis B virus (HBV) replication and IFN-mediated HBV clearance. Methods: We investigated the expression patterns of cellular miRNA induced by HBV replication and/or IFN-alpha treatment in HepG2 cells, and also analyzed the miRNA response in peripheral blood mononuclear cells in CHB patients on IFN-a treatment. The differentially expressed miRNA were verified using quantitative real-time polymerase chain reaction and an miRNA expression pattern was classified based on the final virological response. Results: A total of 223 miRNA were differentially expressed (> 1.5 folds) between the HepG2.2.15 and HepG2 cells, including 24 highly differentially expressed miRNA (> 5 folds). With 12 h of IFN-alpha, treatment, 23 totally differentially expressed miRNA were identified in HepG2 cells; whereas only five miRNA were identified in HepG2.2.15 cells. Similar amounts of the miRNA were regulated in patients with HBeAg or non-HBeAg seroconversion; whereas levels of eight miRNA were significantly differentially expressed between the two groups. Conclusions: HBV replication alters miRNA expression profiles and impairs IFN-inducible miRNA response in HepG2 cells. The miRNA expression pattern of peripheral blood mononuclear cells in CHB patients with ITN therapy can be associated with their therapeutic outcome.
引用
收藏
页码:805 / 812
页数:8
相关论文
共 49 条
  • [21] Upregulated MicroRNA-29a by Hepatitis B Virus X Protein Enhances Hepatoma Cell Migration by Targeting PTEN in Cell Culture Model
    Kong, Guangyao
    Zhang, Junping
    Zhang, Shuai
    Shan, Changliang
    Ye, Lihong
    Zhang, Xiaodong
    [J]. PLOS ONE, 2011, 6 (05):
  • [22] Hepatitis B Virus Regulatory HBx Protein Binds to Adaptor Protein IPS-1 and Inhibits the Activation of Beta Interferon
    Kumar, Manish
    Jung, Sung Yun
    Hodgson, Amanda J.
    Madden, Charles R.
    Qin, Jun
    Slagle, Betty L.
    [J]. JOURNAL OF VIROLOGY, 2011, 85 (02) : 987 - 995
  • [23] Hepatitis B virus X protein prevents apoptosis of hepatocellular carcinoma cells by upregulating SATB1 and HURP expression
    Kuo, Tzu-Ching
    Chao, Chuck C. -K.
    [J]. BIOCHEMICAL PHARMACOLOGY, 2010, 80 (07) : 1093 - 1102
  • [24] Inhibition of STAT1 methylation is involved in the resistance of hepatitis B virus to Interferon alpha
    Li, Jie
    Chen, Feng
    Zheng, Min
    Zhu, Haihong
    Zhao, Dongjiu
    Liu, Weixia
    Liu, Wei
    Chen, Zhi
    [J]. ANTIVIRAL RESEARCH, 2010, 85 (03) : 463 - 469
  • [25] Altered expression profiles of microRNAs in a stable hepatitis B virus-expressing cell line
    Liu Yan
    Zhao Jian-Jun
    Wang Chun-mei
    Li Mian-yang
    Han Ping
    Wang Lin
    Cheng Yong-qian
    Fabien Zoulim
    Ma Xu
    Xu Dong-ping
    [J]. CHINESE MEDICAL JOURNAL, 2009, 122 (01) : 10 - 14
  • [26] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method
    Livak, KJ
    Schmittgen, TD
    [J]. METHODS, 2001, 25 (04) : 402 - 408
  • [27] MicroRNA in the immune system, microRNA as an immune system
    Lu, Li-Fan
    Liston, Adrian
    [J]. IMMUNOLOGY, 2009, 127 (03) : 291 - 298
  • [28] Hepatic microRNA expression is associated with the response to interferon treatment of chronic hepatitis C
    Murakami, Yoshiki
    Tanaka, Masami
    Toyoda, Hidenori
    Hayashi, Katsuyuki
    Kuroda, Masahiko
    Tajima, Atsushi
    Shimotohno, Kunitada
    [J]. BMC MEDICAL GENOMICS, 2010, 3
  • [29] Mechanisms of microRNA-mediated gene regulation in animal cells
    Nilsen, Timothy W.
    [J]. TRENDS IN GENETICS, 2007, 23 (05) : 243 - 249
  • [30] Interferon modulation of cellular microRNAs as an antiviral mechanism
    Pedersen, Irene M.
    Cheng, Guofeng
    Wieland, Stefan
    Volinia, Stefano
    Croce, Carlo M.
    Chisari, Francis V.
    David, Michael
    [J]. NATURE, 2007, 449 (7164) : 919 - U13