Different effects of enhanced and reduced expression of pub gene on the formation of embryoid bodies by cultured embryonic mouse stem cell

被引：3

作者：

Novosadova, EV ^{[1
]}

Manuilova, ES ^{[1
]}

Arsen'eva, EL ^{[1
]}

Khaidarova, NV ^{[1
]}

Dolotov, OV ^{[1
]}

Inozemtseva, LS ^{[1
]}

Kozachenkov, KY ^{[1
]}

Tarantul, VZ ^{[1
]}

Grivennikov, IA ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Mol Genet, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2005年 / 140卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

embryonic stem cells; embryoid bodies; RNA-interference; overexpression; differentiation;

D O I：

10.1007/s10517-005-0432-x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

the effects of pub gene on proliferation and initial stages of differentiation of embryonic Mouse stem cells were studied in vitro. To this end we used enhanced expression of human pub gene (hpub) and suppression of expression of mouse endogenous pub gene with RNA-interference in embryonic stem cells. Proliferative activity of genetically modified polyclonal lines of the embryonic stem cells transfected with plasmids carrying expressing hpub gene or plasmids generating small interference RNA to this gene did not differ from that of the control cells. Inhibition of expression of endogenous pub gene in embryonic stem cells using small interference RNA 2-fold decreased the formation of embryoid bodies, at the same time additional expression of exogenous hpub gene almost 2-fold increased their number in comparison with the control. It was hypothesized that pub gene participates in early stages of differentiation of embryonic stem cells leading to the formation of embryoid bodies.

引用

页码：153 / 158

页数：6

共 17 条

[1] Overexpression of telomerase confers growth advantage, stress resistance, and enhanced differentiation of ESCs toward the hematopoietic lineage [J].