Increased hydrophobicity in Malassezia species correlates with increased proinflammatory cytokine expression in human keratinocytes

被引:36
作者
Akaza, Narifumi [1 ,2 ]
Akamatsu, Hirohiko [3 ]
Takeoka, Shiori [2 ]
Mizutani, Hiroshi [2 ]
Nakata, Satoru [2 ]
Matsunaga, Kayoko [1 ]
机构
[1] Fujita Hlth Univ, Dept Dermatol, Sch Med, Toyoake, Aichi 4701192, Japan
[2] Nippon Menard Cosmet Co Ltd, Res Labs, Nagoya, Aichi, Japan
[3] Fujita Hlth Univ, Dept Appl Cell & Regenerat Med, Sch Med, Toyoake, Aichi 4701192, Japan
关键词
Malassezia; keratinocyte; mRNA; hydrophobicity; beta-1,3-glucan; HUMAN EPIDERMAL-KERATINOCYTES; ATOPIC-DERMATITIS; CANDIDA-ALBICANS; HEALTHY-SUBJECTS; SEBORRHEIC DERMATITIS; PITYROSPORUM-OVALE; IGE ANTIBODIES; CELL-WALL; IN-VITRO; YEAST;
D O I
10.3109/13693786.2012.678019
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Malassezia cells stimulate cytokine production by keratinocytes, although this ability differs among Malassezia species for unknown reasons. The aim of this study was to clarify the factors determining the ability to induce cytokine production by human keratinocytes in response to Malassezia species. M. furfur NBRC 0656, M. sympodialis CBS 7222, M. dermatis JCM 11348, M. globosa CBS 7966, M. restricta CBS 7877, and three strains each of M. globosa, M. restricta, M. dermatis, M. sympodialis, and M. furfur maintained under various culture conditions were used. Normal human epidermal keratinocytes (NHEKs) (1 X 10(5) cells) and the Malassezia species (1 X 10(6) cells) were co-cultured, and IL-1 alpha, IL-6, and IL-8 mRNA levels were determined. Moreover, the hydrophobicity and beta-1,3-glucan expression at the surface of Malassezia cells were analyzed. The ability of Malassezia cells to trigger the mRNA expression of proinflammatory cytokines in NHEKs differed with the species and conditions and was dependent upon the hydrophobicity of Malassezia cells not beta-1,3-glucan expression.
引用
收藏
页码:802 / 810
页数:9
相关论文
共 39 条
[1]  
ADACHI Y, 2007, TRENDS GLYCOSCI GLYC, V19, P195, DOI DOI 10.4052/TIGG.19.195
[2]   Malassezia globosa tends to grow actively in summer conditions more than other cutaneous Malassezia species [J].
Akaza, Narifumi ;
Akamatsu, Hirohiko ;
Takeoka, Shiori ;
Sasaki, Yasuyuki ;
Mizutani, Hiroshi ;
Nakata, Satoru ;
Matsunaga, Kayoko .
JOURNAL OF DERMATOLOGY, 2012, 39 (07) :613-616
[3]   Cutaneous Malassezia microbiota of healthy subjects differ by sex, body part and season [J].
Akaza, Narifumi ;
Akamatsu, Hirohiko ;
Sasaki, Yasuyuki ;
Takeoka, Shiori ;
Kishi, Masataka ;
Mizutani, Hiroshi ;
Sano, Akiyo ;
Hirokawa, Keiko ;
Nakata, Satoru ;
Matsunaga, Kayoko .
JOURNAL OF DERMATOLOGY, 2010, 37 (09) :786-792
[4]   Malassezia folliculitis is caused by cutaneous resident Malassezia species [J].
Akaza, Narifumi ;
Akamatsu, Hirohiko ;
Sasaki, Yasuyuki ;
Kishi, Masataka ;
Mizutani, Hiroshi ;
Sano, Akiyo ;
Hirokawa, Keiko ;
Nakata, Satoru ;
Nishijima, Setsuko ;
Matsunaga, Kayoko .
MEDICAL MYCOLOGY, 2009, 47 (06) :618-624
[5]   Effects of Propionibacterium acnes on various mRNA expression levels in normal human epidermal keratinocytes in vitro [J].
Akaza, Narifumi ;
Akamatsu, Hirohiko ;
Kishi, Masataka ;
Mizutani, Hiroshi ;
Ishii, Izumi ;
Nakata, Satoru ;
Matsunaga, Kayoko .
JOURNAL OF DERMATOLOGY, 2009, 36 (04) :213-223
[6]   CYTOKINE MODULATION OF KERATINOCYTE CYTOKINES [J].
ANSEL, J ;
PERRY, P ;
BROWN, J ;
DAMM, D ;
PHAN, T ;
HART, C ;
LUGER, T ;
HEFENEIDER, S .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1990, 94 (06) :S101-S107
[7]   Toll-like receptor 2 (TLR2) mediates intracellular signalling in human keratinocytes in response to Malassezia furfur [J].
Baroni, A ;
Orlando, M ;
Donnarumma, G ;
Farro, P ;
Iovene, MR ;
Tufano, MA ;
Buommino, E .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 2006, 297 (07) :280-288
[8]   Malassezia Baillon, emerging clinical yeasts [J].
Batra, R ;
Boekhout, T ;
Guého, E ;
Cabañes, FJ ;
Dawson, TL ;
Gupta, AK .
FEMS YEAST RESEARCH, 2005, 5 (12) :1101-1113
[9]   Head and neck atopic dermatitis and Malassezia-furfur-specific IgE antibodies [J].
Bayrou, O ;
Pecquet, C ;
Flahault, A ;
Artigou, C ;
Abuaf, N ;
Leynadier, F .
DERMATOLOGY, 2005, 211 (02) :107-113
[10]   Two new lipid-dependent Malassezia species from domestic animals [J].
Cabanes, F. Javier ;
Theelen, Bart ;
Castella, Gemma ;
Boekhout, Teun .
FEMS YEAST RESEARCH, 2007, 7 (06) :1064-1076