MicroRNA-10b promotes migration and invasion through Hoxd10 in human gastric cancer

被引：40
作者：
Wang, Yuan-Yu [1 ,2 ]
Li, Li [3 ]
Ye, Zai-Yuan [1 ,2 ]
Zhao, Zhong-Sheng [4 ]
Yan, Zhi-Long [1 ,2 ]
机构：
[1] Zhejiang Prov Peoples Hosp, Dept Gastrointestinal Surg, Hangzhou 310014, Zhejiang, Peoples R China
[2] Zhejiang Prov Peoples Hosp, Dept Surg, Hangzhou 310014, Zhejiang, Peoples R China
[3] Key Lab Gastroenterol Zhejiang Prov, Hangzhou 310014, Zhejiang, Peoples R China
[4] Zhejiang Prov Peoples Hosp, Dept Pathol, Hangzhou 310014, Zhejiang, Peoples R China
来源：
WORLD JOURNAL OF SURGICAL ONCOLOGY | 2015年 / 13卷
关键词：
Gastric cancer; miR-10b; Hoxd10; Proliferation; Migration; Invasion; BREAST-CANCER; CELL INVASION; E-CADHERIN; EXPRESSION; INVASIVENESS; METASTASIS; SUPPRESSOR;
D O I：
10.1186/s12957-015-0673-8
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression. Methods: The effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated. Results: miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis. Conclusions: miR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.
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页数：8
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