Clotting of a novel human RNA polymerase II subunit downregulated by doxorubicin: New potential mechanisms of drug related toxicity

被引:20
作者
Fanciulli, M
Bruno, T
Cerboni, C
Bonetto, F
Iacobini, C
Frati, L
Piccoli, M
Floridi, A
Santoni, A
Punturieri, A
机构
[1] REGINA ELENA INST CANC RES,PATHOPHYSIOL LAB,I-00158 ROME,ITALY
[2] UNIV LAQUILA,DEPT EXPTL MED,I-67100 LAQUILA,ITALY
[3] UNIV ROMA LA SAPIENZA,DEPT EXPTL MED & PATHOL,ROME,ITALY
关键词
differential display PCR; doxorubicin; drug toxicity; RNA polymerase II;
D O I
10.1016/0014-5793(96)00277-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using the differential display PCR method, we have isolated an mRNA downregulated in doxorubicin resistant human cell lines, The full length cDNA clone was identified as the human homologue of yeast RPB11 subunit of RNA polymerase II. Northern blot analysis of normal tissues detected a particularly high expression of RPB11 mRNA in heart and skeletal muscle, Reduction of this mRNA expression was observed in all the cell lines tested after drug treatment and was paralleled by a similar decrease of the protein levels, These findings suggest that doxorubicin may exert in vivo specific inhibitory effects on a major component of the transcription machinery.
引用
收藏
页码:48 / 52
页数:5
相关论文
共 22 条
[1]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[2]   INHIBITION OF CHICKEN MYELOBLASTOSIS RNA POLYMERASE-II ACTIVITY BY ADRIAMYCIN [J].
CHUANG, RY ;
CHUANG, LF .
BIOCHEMISTRY, 1979, 18 (10) :2069-2073
[3]   SIMILAR BIOCHEMICAL-CHANGES ASSOCIATED WITH MULTIDRUG RESISTANCE IN HUMAN-BREAST CANCER-CELLS AND CARCINOGEN-INDUCED RESISTANCE TO XENOBIOTICS IN RATS [J].
COWAN, KH ;
BATIST, G ;
TULPULE, A ;
SINHA, BK ;
MYERS, CE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9328-9332
[4]  
DOROSHOW JH, 1983, CANCER RES, V43, P460
[5]  
FORNARI FA, 1994, MOL PHARMACOL, V45, P649
[6]   DOXORUBICIN SELECTIVELY INHIBITS MUSCLE GENE-EXPRESSION IN CARDIAC-MUSCLE-CELLS INVIVO AND INVITRO [J].
ITO, H ;
MILLER, SC ;
BILLINGHAM, ME ;
AKIMOTO, H ;
TORTI, SV ;
WADE, R ;
GAHLMANN, R ;
LYONS, G ;
KEDES, L ;
TORTI, FM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4275-4279
[7]  
KURABAYASHI M, 1993, J BIOL CHEM, V268, P5524
[8]  
KURABAYASHI M, 1994, J BIOL CHEM, V269, P6031
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]   DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].
LIANG, P ;
PARDEE, AB .
SCIENCE, 1992, 257 (5072) :967-971