Assessment of triacylglycerol content in Chlorella vulgaris cultivated in a two-stage process

被引:15
作者
dos Santos, Raquel Rezende [1 ]
Kunigami, Claudete Norie [2 ]
Gomes Aranda, Donato Alexandre [3 ]
Luz Lapa Teixeira, Claudia Maria [1 ]
机构
[1] Inst Nacl Tecnol, Div Energia, Lab Biotecnol Microalgas, BR-20081312 Rio De Janeiro, RJ, Brazil
[2] Inst Nacl Tecnol, Div Quim Anal, Lab Anal Organ Instrumental, BR-20081312 Rio De Janeiro, RJ, Brazil
[3] Univ Fed Rio de Janeiro, Ctr Tecnol, Dept Engn Quim, Lab Tecnol Verde,Escola Quim, BR-21941590 Rio De Janeiro, RJ, Brazil
关键词
Chlorella vulgaris; Biomass productivity; Nitrogen starvation; Triacylglycerol (TAG) content; Two-stage process; NITROGEN-STARVATION; LIPID-ACCUMULATION; BIODIESEL PRODUCTION; WASTE-WATER; MICROALGAE; BIOMASS; GROWTH; CELLS; SPIRULINA; CULTURE;
D O I
10.1016/j.biombioe.2016.05.014
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Chlorella vulgaris cultivation in two-stage process was applied to increase the lipid productivity without compromising the biomass productivity. At the first stage, microalgae was cultivated under nutrient sufficient conditions to obtain a maximized cell density; at the second stage, nitrate conditions are changed to trigger the accumulation of TAG. During first stage, the maximum biomass productivity (32 mg L-1 d(-1)) was observed after 13 days under nutrient sufficient conditions with 1.21 g L-1 NaNO3 and 0.00449 g L-1 K2HPO4. Maximum lipid content (25.4% DW), lipid productivity (7.5 mg L-1 d(-1)) and TAG content (41.3% in total lipids) were favored by the nitrogen starvation conditions for more 4 days, at the second stage. Oil extracted at the second stage contained lower percentage of PUFAs being more suitable for the biodiesel production when compared with the oil extracted at the first stage. This two-stage phototrophic process is promising to provide a more efficient way for on a large-scale production of algal biomass and biodiesel production. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:55 / 60
页数:6
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