Impedance flow cytometry for viability analysis of Corynebacterium glutamicum

被引：5

作者：

Hartmann, Fabian Stefan Franz ^{[1
]}

Anastasiou, Ioannis ^{[1
]}

Weiss, Tamara ^{[1
]}

Shen, Jing ^{[1
]}

Seibold, Gerd Michael ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Biotechnol & Biomed, Sect Synthet Biol, Soltofts Plads Bldg 223, DK-2800 Lyngby, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2021年 / 191卷

关键词：

Corynebacterium glutamicum; Viability; Cell wall inhibitor; Penicillin; Glutamate production; Impedance flow cytometry; L-GLUTAMATE EFFLUX; METABOLISM; BIOMASS;

D O I：

10.1016/j.mimet.2021.106347

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Corynebacterium glutamicum efficiently produces glutamate when growth is inhibited. Analyses of viability in this non-growing state requires time consuming plating and determination of colony forming units. We here establish impedance flow cytometry measurements to assess the viability of non-growing, glutamate producing C. glutamicum cultures within minutes.

引用

页数：4

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