Nanomolar Level Detection of Uric Acid in Blood Serum and Pest-Infested Grain Samples by an Amphiphilic Probe

被引：71

作者：

Dey, Nilanjan ^{[1
]}

Bhattacharya, Santanu ^{[1
,2
]}

机构：

[1] Indian Inst Sci, Dept Organ Chem, Bangalore 560012, Karnataka, India

[2] Indian Assoc Cultivat Sci, Directors Res Unit, Kolkata 700032, India

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 19期

关键词：

AGGREGATION-INDUCED EMISSION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; POSITIONAL ISOMERS; BIOLOGICAL-FLUIDS; VESICLE FORMATION; WATER; FLUORESCENCE; EXCIMER; HYPERURICEMIA;

D O I：

10.1021/acs.analchem.7b02344

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A pyrene based amphiphilic receptor has been utilized in the nanomolar detection of uric acid at physiological pH in water. The compound shows a unique concentration-dependent modulation in optical response toward uric acid. In intramolecular/static excimer form (low concentration range), it displays a ratiometric response, while a "turn-off' sensing is observed specifically in the presence of intermolecular/dynamic excimer (high concentration range). The present protocol is then employed for the estimation of uric acid in blood serum samples of healthy individuals. Bland-Altman analysis in comparison to clinically approved uricase assay indicated the high accuracy of the present method. Additionally, the extent of insect infestation in stored grain samples was determined by measuring the uric acid content of their aqueous extracts. Low-cost color strips were developed for on-site detection of uric acid without involving any sophisticated instrument or trained personnel.

引用

页码：10376 / 10383

页数：8

共 65 条

[1] Uric acid: role in cardiovascular disease and effects of losartan [J].

Alderman, M ;

Aiyer, KJV .

CURRENT MEDICAL RESEARCH AND OPINION, 2004, 20 (03) :369-379

[2] A New Selective Chromogenic and Turn-On Fluorogenic Probe for Copper(II) in Water-Acetonitrile 1:1 Solution [J].

Ballesteros, Eduardo ;

Moreno, Daniel ;

Gomez, Teresa ;

Rodriguez, Teresa ;

Rojo, Josefa ;

Garcia-Valverde, Maria ;

Torroba, Tomas .

ORGANIC LETTERS, 2009, 11 (06) :1269-1272

[3] Aggregation, binding, and dimerisation studies of a teicoplanin aglycone analogue (LY154989) [J].

Bardsley, B ;

Zerella, R ;

Williams, DH .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 2002, (03) :598-603

[4] TOWARDS THE PHYSIOLOGICAL-FUNCTION OF URIC-ACID [J].

BECKER, BF .

FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (06) :615-631

[5] Hyperuricemia and associated diseases [J].

Becker, Michael A. ;

Jolly, Meenakshi .

RHEUMATIC DISEASE CLINICS OF NORTH AMERICA, 2006, 32 (02) :275-+

[6] A SPECTROPHOTOMETRIC INVESTIGATION OF THE INTERACTION OF IODINE WITH AROMATIC HYDROCARBONS [J].

BENESI, HA ;

HILDEBRAND, JH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1949, 71 (08) :2703-2707

[7] Enzyme-free colorimetric assay of serum uric acid [J].

Bera, Raj Kumar ;

Anoop, Anakuthil ;

Raj, C. Retna .

CHEMICAL COMMUNICATIONS, 2011, 47 (41) :11498-11500

[8] Evidence of enhanced reactivity of DAAP nucleophiles toward dephosphorylation and deacylation reactions in cationic gemini micellar media [J].

Bhattacharya, S ;

Kumar, VP .

JOURNAL OF ORGANIC CHEMISTRY, 2004, 69 (02) :559-562

[9] Synthesis and vesicle formation from novel pseudoglyceryl dimeric lipids. Evidence of formation of widely different membrane organizations with exceptional thermotropic properties [J].

Bhattacharya, S ;

De, S ;

George, SK .

CHEMICAL COMMUNICATIONS, 1997, (23) :2287-2288

[10]

Bhattacharya S, 1999, CHEM-EUR J, V5, P2335, DOI 10.1002/(SICI)1521-3765(19990802)5:8<2335::AID-CHEM2335>3.0.CO

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