Coupling of short DNAs with reduced graphene oxide for electronic and sensing applications

被引：3

作者：

Komarov, I. A. ^{[1
]}

Antipova, O. M. ^{[2
]}

Kalinnikov, A. N. ^{[1
]}

Orlov, M. A. ^{[1
]}

Bogachev, V. V. ^{[1
]}

Buyanov, A. D. ^{[1
]}

Onoprienko, E. A. ^{[1
]}

机构：

[1] Bauman Moscow State Tech Univ, Interbranch Engn Ctr Composites Russia, Moscow, Russia

[2] Lomonosov Moscow State Univ, Chem Dept, Moscow, Russia

来源：

FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES | 2020年 / 28卷 / 07期

关键词：

Aptamer; biosensor; covalent coupling; reduced graphene oxide; REDUCTION; BIOSENSOR; FILMS; PHOTOREDUCTION; STABILITY; ACID;

D O I：

10.1080/1536383X.2020.1713761

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this article, we described an approach for coupling of short DNAs with reduced graphene oxide and thus formation of transducer layer for biological sensors. We investigated the dependence of coupling ratio on the graphene oxide reduction level. We found optimal reduction parameters and showed successful conjugation of aptamers with reduced graphene oxide. We have revealed a trend to increase aptamer conjugation efficiency with a decrease of graphene oxide reduction rate. Finally, we made biosensor structures with pi-shaped reduction pattern and showed excellent sensitivity of the sensor during thrombin exposure. These results are important for the development of flexible low-cost biosensors of a new generation.

引用

页码：526 / 532

页数：7

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