MiR-424-5p promotes cell invasion and migration by targeting CYLD in human pancreatic cancer

The effect and mechanism of miR-424-5p on the invasion and migration of human pancreatic cancer is unknown. Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of miR-424-5p and CYLD in pancreatic cancer specimens and pancreatic cancer cells PANC-1. MiR-424-5p mimics/inhibitors and their negative controls were transfected into PANC-1 cells respectively, following by transwell, and wound healing assays, which were used for investigating the capacity of cell invasion and migration. Meanwhile, protein levels of CYLD, Smad3 and E-cadherin were examined through western blotting. Luciferase reporter assay was used to validate CYLD as a target of miR-424-5p. MiR-424-5p suppressed the expression of CYLD and was significantly upregulated in pancreatic cancer specimens and cells. Overexpression of miR-424-5p promoted cell invasion and migration, which on the contrary would be reduced by the downregulation of miR-424-5p in vitro. MiR-424-5p may increase pancreatic cancer cells invasion and migration by targeting CYLD, and thus potentially serving as a new therapeutic target for pancreatic cancer.