ADVENTITIOUS SHOOT REGENERATION OF DWARF HYGRO (Hygrophila polysperma) UNDER IN VITRO CONDITIONS

被引:0
作者
Karatas, Mehmet [1 ]
Aasim, Muhammad [1 ]
Cinar, Aysegul [1 ]
机构
[1] Karamanoglu Mehmetbey Univ, Kamil Ozdag Fac Sci, Dept Biol, TR-70200 Karaman, Turkey
来源
FRESENIUS ENVIRONMENTAL BULLETIN | 2014年 / 23卷 / 09期
关键词
Adventitious; dwarf hygro; in vitro; leaf explant; liquid culture; shoot regeneration; BACOPA-MONNIERI L; PLANTLET REGENERATION; LIQUID CULTURES; MICROPROPAGATION; EXPLANTS; ORGANOGENESIS; CONTAMINATION; PROPAGATION; TIP;
D O I
暂无
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Dwarf hygro (Hygrophila polysperma [Roxb.] T. Anderson) is an important medicinal aquatic plant and bioindicator. The study presents in vitro adventitious shoot regeneration from leaf explant of dwarf hygro cultured in liquid MS medium containing 0.50-2.50 mg/l Benzylaminopurine (BA). Adventitious shoot regeneration initiated from leaf margins and maximum of 5.11 shoots per explant were induced on MS medium containing 1.0 mg/l BA. After 6 weeks of culture. explants were transferred to culture medium supplemented with 0.502.50 mg/l BA plus 1.0 mg/l Gibberellic acid (GA(3)) for shoot elongation and multiplication. Addition of GA(3) along with different concentrations of BA increased the shoot number and shoot length. Maximum of 10.92 shoot were recorded from MS medium containing 1.0 mg/l BA+1.0 mg/l GA(3). Whereas, longer shoot (1.24 cm) was recorded from 0.5 mg/l BA-1.0 mg/l GA(3) Regenerated shoots were rooted successfully on agar solidified MS medium containing 0.10-1.00 mg/l Indole-3-butyric acid (IBA) and 100% rooting was achieved within 3 weeks. Rooted plantlets were acclimatized in aquarium containing tap water where they continue their growth.
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页码:2190 / 2194
页数:5
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