Citrullus colocynthis extract and synthesized Selenium nanoparticles enhance non-specific response and resistance against Aeromonas sobria in Nile tilapia (Oreochromis niloticus )

被引:12
作者
Ayoub, Hala F. [1 ]
Tohamy, Eman Y. [2 ]
Salama, Hediat M. [2 ]
Mohamed, Sara S. [2 ]
机构
[1] Agr Res Ctr, Dept Fish Hlth & Management, Cent Lab Aquaculture Res CLAR, Abbassa, Abo Hammad, Egypt
[2] Zagazig Univ, Dept Bot & Microbiol, Fac Sci, Zagazig, Egypt
关键词
Aeromonas sobria; antioxidants; Citrullus colocynthis; innate immunity; medicinal herbs; Nile tilapia; Se nanoparticles; TRADITIONAL CHINESE MEDICINE; DIETARY SELENIUM; OXIDATIVE STRESS; GROWTH-PERFORMANCE; SUPEROXIDE-DISMUTASE; PATHOLOGICAL-CHANGES; DISEASE RESISTANCE; ANTIOXIDANT STATUS; MUSCLE COMPOSITION; IMMUNE-RESPONSES;
D O I
10.1111/are.15366
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The effects of Citrullus colocynthis extract (CCE) and synthesized Se nanoparticles (Nano-Se) on non-specific immune response and resistance to Aeromonas sobria in Nile tilapia (Oreochromis niloticus) were investigated. Treatments included three levels of Nano-Se (0.5, 1 and 2 mg/kg of feed), three levels of CCE (0.1%, 0.3% and 0.5%) and control group (no additives). The diets were fed for 4 weeks, and biochemical indices, serum lysozyme, respiratory burst activities and antioxidant enzymes were measured on weeks 1, 2 and 4. After 4 weeks of feeding, fish were infected with A. sobria and mortalities were recorded for 14 days. Results of this study showed that feeding tilapia with CCE and Nano-Se supplements significantly enhanced biochemical indices, serum lysozymes and respiratory burst. CCE treatments had a moderate effect on superoxide dismutase and no effect on the other antioxidant enzymes. Nano-Se treatments had significantly positive effects on antioxidant enzymes. Both CCE and Nano-Se supplements reduced mortality following A. sobria infection. The lowest mortality (13%) was observed in the group fed with 2 mg/kg Nano-Se, and the highest mortality of 90% was observed in the control group. It can be concluded that C. colocynthis extract and synthesized Nano-Se supplementation added to fish feed can act as immunostimulants and enhance the immune functionality, antioxidant status and disease resistance in cultured Nile tilapia (O. niloticus).
引用
收藏
页码:4969 / 4982
页数:14
相关论文
共 75 条
  • [1] Evaluation of the hematoprotective and hepato-renal protective effects of Thymus vulgaris aqueous extract on thermally oxidized oil-induced hematotoxicity and hepato-renal toxicity
    Abdallah A.A.M.
    Nasr El-Deen N.A.M.
    Neamat-Allah A.N.F.
    Abd El-Aziz H.I.
    [J]. Comparative Clinical Pathology, 2020, 29 (2) : 451 - 461
  • [2] Lycopene and resveratrol ameliorate zinc oxide nanoparticles-induced oxidative stress in Nile tilapia, Oreochromis niloticus
    Abdel-Daim, Mohamed M.
    Eissa, Ismail A. M.
    Abdeen, Ahmed
    Abdel-Latif, Hany M. R.
    Ismail, Mona
    Dawood, Mahmoud A. O.
    Hassan, Ahmed M.
    [J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2019, 69 : 44 - 50
  • [3] Dietary origanum essential oil improved antioxidative status, immune-related genes, and resistance of common carp (Cyprinus carpio L.) to Aeromonas hydrophila infection
    Abdel-Latif, Hany M. R.
    Abdel-Tawwab, Mohsen
    Khafaga, Asmaa F.
    Dawood, Mahmoud A. O.
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2020, 104 : 1 - 7
  • [4] AEBI H, 1984, METHOD ENZYMOL, V105, P121
  • [5] Biochemical effects of Citrullus colocynthis in normal and diabetic rats
    Al-Ghaithi, F
    El-Ridi, MR
    Adeghate, E
    Amiri, MH
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2004, 261 (1-2) : 143 - 149
  • [6] Ambi A.A., 2007, NIGERIAN J PHARM SCI, V6, P7
  • [7] Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila
    Ardo, Laszlo
    Yin, Guojun
    Xu, Pao
    Varadi, Laszlo
    Szigeti, Gabor
    Jeney, Zsigmond
    Jeney, Galina
    [J]. AQUACULTURE, 2008, 275 (1-4) : 26 - 33
  • [8] Effects of dietary selenium on the oxidative stress and pathological changes in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom
    Atencio, Loyda
    Moreno, Isabel
    Jos, Angeles
    Prieto, Ana I.
    Moyano, Rosario
    Blanco, Alfonso
    Camean, Ana M.
    [J]. TOXICON, 2009, 53 (02) : 269 - 282
  • [9] Ayoub H.F., 2019, Life Sci. J, V16, P8, DOI DOI 10.7537/MARSLSJ160419.02
  • [10] Badawi M.E., 2016, Japanese Journal of Veterinary Research, V64, pS87