In Situ Spectroscopic Ellipsometry Study of Protein Immobilization on Different Substrates Using Liquid Cells

被引：12
|
作者：
Nemeth, Andrea [1 ]
Kozma, Peter [1 ,2 ]
Huelber, Timea [1 ]
Kurunczi, Sandor [1 ]
Horvath, Robert [1 ]
Petrik, Peter [1 ]
Muskotal, Adel [2 ]
Vonderviszt, Ferenc [1 ,2 ]
Hos, Csaba [3 ]
Fried, Miklos [1 ]
Gyulai, Jozsef [1 ]
Barsony, Istvan [1 ]
机构：
[1] Hungarian Acad Sci, Res Inst Tech Phys & Mat Sci, H-1525 Budapest, Hungary
[2] Univ Pannonia, Dept Nanotechnol, Res Inst Chem & Proc Engn, Fac Informat Technol, H-8200 Veszprem, Hungary
[3] Budapest Univ Technol & Econ, Dept Hydrodinam Syst, H-1111 Budapest, Hungary
来源：
SENSOR LETTERS | 2010年 / 8卷 / 05期
关键词：
Protein Adsorption; Biosensor; Spectroscopic Ellipsometry; Liquid Cell; FIBRINOGEN ADSORPTION; TIME; SURFACES; LAYERS;
D O I：
10.1166/sl.2010.1338
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The influence of substrate materials on protein adsorption was studied by spectroscopic ellipsometry (SE) and atomic force microscopy. For model proteins fibrinogen and flagellar filaments were chosen and their kinetics of adsorption, surface coverage and adsorbed amount on virgin and chemically activated SiO2 and Ta2O5 thin films were investigated. In case of flagellar filaments the SE data were analyzed with an effective medium model that accounted for the vertical density distribution of the adsorbed protein layer. Adsorption was measured in situ using flow cells with various fluid volume. Compared to commercially available cells, a flow cell with significantly smaller volume was constructed for cost-effective measurements. The development of the flow cell was supported by finite element fluid dynamics calculations.
引用
收藏
页码：730 / 735
页数：6
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