High prevalence, genetic diversity and a potentially novel genotype of Sapelovirus A (Picornaviridae) in enteric and respiratory samples in Hungarian swine farms

被引：19

作者：

Boros, Akos ^{[1
]}

Laszlo, Zoltan ^{[1
]}

Pankovics, Peter ^{[1
]}

Marosi, Andras ^{[2
]}

Albert, Mihaly ^{[3
]}

Csagola, Attila ^{[3
]}

Biro, Hunor ^{[4
]}

Fahsbender, Elizabeth ^{[5
]}

Delwart, Eric ^{[5
,6
]}

Reuter, Gabor ^{[1
]}

机构：

[1] Univ Pecs, Med Sch, Dept Med Microbiol & Immunol, Pecs, Hungary

[2] Univ Vet Med, Dept Microbiol & Infect Dis, Budapest, Hungary

[3] Ceva Phylaxia Ltd, Budapest, Hungary

[4] SHP Ltd, Kaposvar, Hungary

[5] Vitalant Res Inst, San Francisco, CA USA

[6] Univ Calif San Francisco, San Francisco, CA 94143 USA

来源：

JOURNAL OF GENERAL VIROLOGY | 2020年 / 101卷 / 06期

基金：

匈牙利科学研究基金会;

关键词：

swine; picornavirus; sapelovirus; extraintestinal; isolation; paraplegia; PORCINE ENTEROVIRUSES; VIRUS; CLASSIFICATION; PATHOGENICITY; INFECTION; ELEMENTS; TISSUE; ACID;

D O I：

10.1099/jgv.0.001410

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

All of the known porcine sapeloviruses (PSVs) currently belong to a single genotype in the genus Sapelovirus (family Picornaviridae). Here, the complete genome of a second, possibly recombinant, genotype of PSV strain SZ1M-F/PSV/HUN2013 (MN807752) from a faecal sample of a paraplegic pig in Hungary was characterized using viral metagenomics and RT-PCR. This sapelovirus strain showed only 64 % nucleotide identity in the VP1 region to its closest PSV-1 relative. Complete VP1 sequence-based epidemiological investigations of PSVs circulating in Hungary showed the presence of diverse strains found in high prevalence in enteric and respiratory samples collected from both asymptomatic and paraplegic pigs from 12 swine farms. Virus isolation attempts using PK-15 cell cultures were successful in 3/8 cases for the classic but not the novel PSV genotype. Sequence comparisons of faeces and isolate strains derived VP1 showed that cultured PSV strains not always represent the dominant PSVs found in vivo.

引用

页码：609 / 621

页数：13

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