Ammonium and nitrite oxidation at nanomolar oxygen concentrations in oxygen minimum zone waters

被引:201
作者
Bristow, Laura A. [1 ,7 ]
Dalsgaard, Tage [2 ]
Tiano, Laura [3 ]
Mills, Daniel B. [1 ]
Bertagnolli, Anthony D. [4 ,5 ]
Wright, Jody J. [6 ]
Hallam, Steven J. [6 ]
Ulloa, Osvaldo [4 ,5 ]
Canfield, Donald E. [1 ]
Revsbech, Niels Peter [3 ]
Thamdrup, Bo [1 ]
机构
[1] Univ Southern Denmark, Dept Biol, Nord Ctr Earth Evolut, DK-5230 Odense M, Denmark
[2] Aarhus Univ, Arctic Res Ctr, Dept Biosci, DK-8000 Aarhus C, Denmark
[3] Aarhus Univ, Dept Biosci, Microbiol, DK-8000 Aarhus C, Denmark
[4] Univ Concepcion, Dept Oceanog, Concepcion 4070386, Chile
[5] Univ Concepcion, Inst Milenio Oceanog, Concepcion 4070386, Chile
[6] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
[7] Max Planck Inst Marine Microbiol, Biogeochem Dept, D-28359 Bremen, Germany
基金
加拿大创新基金会; 加拿大自然科学与工程研究理事会; 欧洲研究理事会; 新加坡国家研究基金会;
关键词
oxygen; ammonium oxidation; nitrite oxidation; OMZ; kinetics; COASTAL UPWELLING ECOSYSTEM; OXIDIZING ARCHAEA; DEFICIENT WATERS; AEROBIC RESPIRATION; NITROGEN-CYCLE; FRESH-WATER; MARINE; DENITRIFICATION; BACTERIA; NITRIFICATION;
D O I
10.1073/pnas.1600359113
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A major percentage of fixed nitrogen (N) loss in the oceans occurs within nitrite-rich oxygen minimum zones (OMZs) via denitrification and anammox. It remains unclear to what extent ammonium and nitrite oxidation co-occur, either supplying or competing for substrates involved in nitrogen loss in the OMZ core. Assessment of the oxygen (O-2) sensitivity of these processes down to the O-2 concentrations present in the OMZ core (< 10 nmol.L-1) is therefore essential for understanding and modeling nitrogen loss in OMZs. We determined rates of ammonium and nitrite oxidation in the seasonal OMZ off Concepcion, Chile at manipulated O-2 levels between 5 nmol.L-1 and 20 mu mol.L-1. Rates of both processes were detectable in the low nanomolar range (5-33 nmol.L-1 O-2), but demonstrated a strong dependence on O-2 concentrations with apparent half-saturation constants (K(m)s) of 333 +/- 130 nmol.L-1 O-2 for ammonium oxidation and 778 +/- 168 nmol.L-1 O-2 for nitrite oxidation assuming one-component Michaelis-Menten kinetics. Nitrite oxidation rates, however, were better described with a two-component Michaelis-Menten model, indicating a high-affinity component with a Km of just a few nanomolar. As the communities of ammonium and nitrite oxidizers were similar to other OMZs, these kinetics should apply across OMZ systems. The high O-2 affinities imply that ammonium and nitrite oxidation can occur within the OMZ core whenever O-2 is supplied, for example, by episodic intrusions. These processes therefore compete with anammox and denitrification for ammonium and nitrite, thereby exerting an important control over nitrogen loss.
引用
收藏
页码:10601 / 10606
页数:6
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