In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

被引：20

作者：

An, Jun ^{[1
,2
,3
]}

Yang, Cui ^{[1
,2
]}

Li, Zuming ^{[1
,2
]}

Finn, Patricia W. ^{[4
]}

Perkins, David L. ^{[4
]}

Sun, Jun ^{[4
]}

Bai, Zhihui ^{[5
]}

Gao, Liping ^{[1
,2
]}

Zhang, Michael ^{[6
]}

Ren, Difeng ^{[3
]}

机构：

[1] Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Beijing, Peoples R China

[2] Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China

[3] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing Key Lab Forest Food Proc & Safety, Beijing 100083, Peoples R China

[4] Univ Illinois, Dept Med, Chicago, IL USA

[5] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China

[6] Univ Manitoba, Dept Phys & Astron, Winnipeg, MB R3T 2N2, Canada

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2019年 / 103卷 / 02期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

Rhodobacter sphaeroides; Antioxidant activities; Oxidative stress; Caco-2; cell; PROBIOTIC PROPERTIES; OXIDATIVE STRESS; FREE-RADICALS; BACTERIA; PEPTIDES; FRACTIONATION; ENHANCEMENT; EXTRACTION; ASSAY;

D O I：

10.1007/s00253-018-9497-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cells and intracellular cell-free extracts showed better antioxidant activities. Caco-2 cell-based oxidative stress model was developed by optimizing H2O2 concentration and culture time with the half lethal dose and methyl thiazolyl tetrazolium. By increasing the activity of endogenous antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, upregulating the antioxidant ability of the anti-superoxide anion and anti-hydroxyl radical, R. sphaeroides, especially the mutant strain R. sphaeroides (CGMCC No. 8513), exhibited significant protective activity against H2O2-induced oxidative stress in Caco-2 cells. Taken together, R. sphaeroides (CGMCC No. 8513) exhibits strong antioxidant activities and is a candidate to be investigated as a potential probiotic in the future.

引用

页码：917 / 927

页数：11

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