A sandwich ELISA for detecting the hemagglutinin of avian influenza A (H10N8) virus

被引：7
作者：
Chen, Lingling [1 ,2 ]
Ruan, Feier [1 ,2 ]
Liu, Mingbin [3 ,4 ]
Zhou, Jianfang [2 ]
Song, Wenjun [5 ]
Qin, Kun [2 ]
机构：
[1] Nanchang Univ, Sch Publ Hlth, Jiangxi Prov Key Lab Prevent Med, Nanchang, Jiangxi, Peoples R China
[2] China CDC, Key Lab Med Virol, Natl Hlth Commiss PR China, Natl Inst Viral Dis Control & Prevent, 100 Yingxin St, Beijing 100052, Peoples R China
[3] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
[4] Nanchang Ctr Dis Control & Prevent, Nanchang, Jiangxi, Peoples R China
[5] Guangzhou Med Univ, Inst Integrat Tradit & Western Med, State Key Lab Resp Dis, Guangzhou, Guangdong, Peoples R China
来源：
JOURNAL OF MEDICAL VIROLOGY | 2019年 / 91卷 / 05期
基金：
中国国家自然科学基金;
关键词：
H10; subtype; hemagglutinin; monoclonal antibody; sandwich ELISA; LIVE POULTRY MARKETS; HUMAN INFECTION;
D O I：
10.1002/jmv.25387
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Novel influenza A virus (H10N8) infected human with fatality in China during 2013-2014. It is important to detect such nonprevalent subtype influenza A virus in clinic and regular surveillance in the early stage for effective control and prevention from the potential pandemic. Unavailability of convenient rapid diagnosis for this subtype virus in resources-limited setting is an obstacle for timely recognizing human case. In the present study, a panel of mouse H10 specific monoclonal antibodies (mAbs) was generated, two of which were used to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting the hemagglutinin of avian influenza A (H10N8) virus. ELISA results showed high sensitivity with the lowest detection limit of 0.5HAU/50 mu L for live virus, which laid a foundation for clinic use as a promising diagnostic methodology.
引用
收藏
页码：877 / 880
页数：4
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