Polishing of screen-printed electrodes improves IgG adsorption

被引:20
作者
Pravda, M [1 ]
O'Meara, C [1 ]
Guilbault, GG [1 ]
机构
[1] Natl Univ Ireland Univ Coll Cork, Dept Chem, Cork, Ireland
关键词
electrode polishing; screen-printed electrode; protein adsorption; IgG; biosensors; alkaline phosphatase; p-aminophenyl phosphate;
D O I
10.1016/S0039-9140(01)00347-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The screen-printing technology has several advantages over the others for industrial sensor production. In fact, it has the advantage of its heterogeneic nature, which is very similar to carbon paste electrodes, combined with the properties of solid electrodes. Carbon ink may be modified with metallic particles, mediators, biocomponents, etc. Carbon screen-printed electrode (SPE) may be coated with additional layers by again screen-printing, adsorption, electropolymerization, etc. The aim of this work is to investigate the effect of polishing the carbon SPE for the active electrode area, for adsorption properties, and for reproducibility. Two polishing methods were investigated, a gentle method using an office paper, and an aggressive one using an emery paper (SiC 240 mum). The adsorption of rabbit immunoglobulin (IgG) was significantly enhanced in the order, emery paper > office paper > original. The reproducibility between electrodes was found to be in this order, office paper > emery paper > original. The electrode area was not significantly affected by any polishing. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:887 / 892
页数:6
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