Incorporation of electroendocytosis and nanosecond pulsed electric field in electrochemotherapy of breast cancer cells

被引：4
作者：
Abd-Elghany, Amr A. [1 ,2 ]
机构：
[1] Prince Sattam Bin Abdul Aziz Univ, Coll Appl Med Sci, Radiol & Med Imaging Dept, Alkharj, Saudi Arabia
[2] Cairo Univ, Biophys Dept, Fac Sci, Giza, Egypt
来源：
ELECTROMAGNETIC BIOLOGY AND MEDICINE | 2022年 / 41卷 / 01期
关键词：
Low-electric field (LEF) monopolar pulses; nanosecond pulsed electric fields (nsPEF); cloning efficacy test; bleomycin (BLM); MCF-7; cells; RECEPTOR-MEDIATED ENDOCYTOSIS; FREQUENCY; ELECTROPORATION; PROTEIN;
D O I：
10.1080/15368378.2021.1978479
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The study aimed to evaluate the possibility to perform electrochemotherapy using nanosecond pulsed electric field (nsPEF) and low electric field (LEF) monopolar electrical impulses to alleviate the problems of conventional electroporation. Two types of pulses have been used to treat MCF-7 human breast carcinoma cell line: very low voltage (electric field strength) long trains of short unipolar electric pulses, and low frequencies of extremely intense (40kV/cm), ultra-short (10ns) electric pulses. The electropermeabilization efficiency of the formed endocytotic vesicles was measured using the cloning efficacy test. The cell viability was decreased significantly at a repetition frequency begins from 0.01 Hz by similar to 35% and reached complete cell loss at 1 Hz of nanosecond pulses for cells treated before with monopolar pulses at 20 V/cm in the presence of BLM with 4 mu M concentration. The uptake of non-permeant drugs has been done without plasma membrane permeabilization (classical electroporation), but by endocytosis. Nanosecond electric pulses can disrupt the membrane of endocytotic vesicles and release the cytotoxic drug bleomycin.
引用
收藏
页码：25 / 34
页数：10
相关论文
共 50 条
[41]   Chip Scale Tunable Nanosecond Pulsed Electric Field Generator for Cell Electroporation [J].
Kadja, Tchamie ;
Kota, Akash ;
Chodavarapu, Vamsy P. .
PROCEEDINGS OF THE 2019 IEEE NATIONAL AEROSPACE AND ELECTRONICS CONFERENCE (NAECON), 2019, :465-468
[42]   Effect of cytoskeleton in tumor cell apoptosis induced by nanosecond pulsed electric field [J].
Guo, Fei ;
Yao, Chen-Guo ;
Zhang, Xi-Ming ;
Sun, Cai-Xin ;
Xiao, De-You ;
Tang, Li-Ling .
Gaodianya Jishu/High Voltage Engineering, 2012, 38 (01) :205-210
[43]   In Vivo Experimental Study of Nanosecond Pulsed Electric Field Effects on Solid Tumors [J].
Nagahama, Masataka ;
Shimomura, Naoyuki ;
Nakagawa, Akito ;
Teranishi, Kenji ;
Uto, Yoshihiro ;
Hori, Hitoshi .
IEEE TRANSACTIONS ON DIELECTRICS AND ELECTRICAL INSULATION, 2013, 20 (04) :1266-1272
[44]   Histopathology of normal skin and melanomas after nanosecond pulsed electric field treatment [J].
Chen, Xinhua ;
Swanson, R. James ;
Kolb, Juergen F. ;
Nuccitelli, Richard ;
Schoenbach, Karl H. .
MELANOMA RESEARCH, 2009, 19 (06) :361-371
[45]   Microdosimetry for Nanosecond Pulsed Electric Field Applications: A Parametric Study for a Single Cell [J].
Merla, Caterina ;
Paffi, Alessandra ;
Apollonio, Francesca ;
Leveque, Philippe ;
d'Inzeo, Guglielmo ;
Liberti, Micaela .
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2011, 58 (05) :1294-1302
[46]   Cancer Treatment: An Overview of Pulsed Electric Field Utilization and Generation [J].
Alshahat, Mahmoud A. ;
Elgenedy, Mohamed A. ;
Aboushady, Ahmed A. ;
Williams, Mark T. S. .
APPLIED SCIENCES-BASEL, 2023, 13 (18)
[47]   A Novel Material Incorporation Technique for Medaka (Oryzias latipes) Eggs Using Nanosecond Pulsed Electric Fields [J].
Tominaga, Nobuaki ;
Kono, Susumu ;
Yamaguchi, Akemi ;
Akiyama, Hidenori ;
Arizono, Koji .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2010, 74 (06) :1279-1282
[48]   Caspase dependent apoptosis induced in yeast cells by nanosecond pulsed electric fields [J].
Simonis, Povilas ;
Kersulis, Skirmantas ;
Stankevich, Voitech ;
Kaseta, Vytautas ;
Lastauskiene, Egle ;
Stirke, Arunas .
BIOELECTROCHEMISTRY, 2017, 115 :19-25
[49]   Electrode microchamber for noninvasive perturbation of mammalian cells with nanosecond pulsed electric fields [J].
Sun, YH ;
Vernier, PT ;
Behrend, M ;
Marcu, L ;
Gundersen, MA .
IEEE TRANSACTIONS ON NANOBIOSCIENCE, 2005, 4 (04) :277-283
[50]   Differential Sensitivities of Malignant and Normal Skin Cells to Nanosecond Pulsed Electric Fields [J].
Yang, W. ;
Wu, Y-H. ;
Yin, D. ;
Koeffler, H. P. ;
Sawcer, D. E. ;
Vernier, P. T. ;
Gundersen, M. A. .
TECHNOLOGY IN CANCER RESEARCH & TREATMENT, 2011, 10 (03) :281-286
12345