Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification

被引：127

作者：

Liu, Chi-Jen ^{[1
]}

Wang, Chang-Hai ^{[1
]}

Chien, Chia-Chi ^{[1
,2
]}

Yang, Tsung-Yeh ^{[1
]}

Chen, Shin-Tai ^{[1
]}

Leng, Wei-Hua ^{[1
]}

Lee, Cheng-Feng ^{[1
]}

Lee, Kuen-Ho ^{[1
]}

Hwu, Y. ^{[1
,2
,3
,4
]}

Lee, Yao-Chang ^{[4
]}

Cheng, Chia-Liang ^{[5
]}

Yang, Chung-Shi ^{[6
]}

Chen, Y. J. ^{[7
]}

Je, J. H. ^{[8
]}

Margaritondo, G. ^{[9
]}

机构：

[1] Acad Sinica, Inst Phys, Taipei 115, Taiwan

[2] Natl Tsing Hua Univ, Dept Engn & Syst Sci, Hsinchu 300, Taiwan

[3] Natl Taiwan Ocean Univ, Inst Optoelect Sci, Chilung 202, Taiwan

[4] Natl Synchrotron Radiat Res Ctr, Hsinchu 30076, Taiwan

[5] Natl Dong Hwa Univ, Dept Phys, Hualien 97401, Taiwan

[6] Natl Hlth Res Inst, Ctr Nanomed Res, Miaoli 350, Taiwan

[7] Mackay Mem Hosp, Dept Radiat Oncol & Med Res, Taipei 104, Taiwan

[8] Pohang Univ Sci & Technol, Xray Imaging Ctr, Pohang 790784, South Korea

[9] Ecole Polytech Fed Lausanne, CH-1015 Lausanne, Switzerland

来源：

NANOTECHNOLOGY | 2008年 / 19卷 / 29期

关键词：

D O I：

10.1088/0957-4484/19/29/295104

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We explored a very interesting gold nanoparticle system-pegylated gold in colloidal solution-and analyzed its uptake by mice colorectal adenocarcinoma CT26 tumor cells and the impact on the cell's response to x-ray irradiation. We found that exposure to polyethylene glycol ( PEG) modified ('pegylated') 4.7 +/- 2.6 nm gold nanoparticles synthesized by a novel synchrotron-based method enhances the response of CT26 cells to x-ray irradiation. Transmission electron microscopy (TEM) and confocal microscopy revealed that substantial amounts of such nanoparticles are taken up and absorbed by the cells and this conclusion is supported by quantitative induced coupled plasma (ICP) results. Standard tests indicated that the internalized particles are highly biocompatible but strongly enhance the cell damage induced by x-ray irradiation. Synchrotron radiation Fourier transform infrared (SR-FTIR) spectromicroscopy analyzed the chemical aspects of this phenomenon: the appearance of C = O stretching bond spectral features could be used as a marker for cell damage and confirmed the enhancement of the radiation-induced toxicity for cells.

引用

页数：5

共 22 条

[1] Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells [J].