Electrochemical impedance spectroscopy sensor for ascorbic acid based on copper(I) catalyzed click chemistry

被引：57
作者：
Qiu, Suyan [1 ]
Gao, Sen [1 ]
Liu, Qida [2 ]
Lin, Zhenyu [1 ]
Qiu, Bin [1 ]
Chen, Guonan [1 ]
机构：
[1] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
[2] Xi An Jiao Tong Univ, MOE Key Lab Strength & Vibrat, Xian 710049, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 11期
关键词：
Click chemistry; Electrochemical impedance spectroscopy; Copper(I) catalyzed azides and alkynes cycloaddition; Fractional surface coverage (theta); Ascorbic acid; SILVER NANOPARTICLES; ELECTRODE SURFACES; GOLD NANOPARTICLES; VISUAL DETECTION; FERROCENE; ALKYNE; MONOLAYERS; PLATFORM;
D O I：
10.1016/j.bios.2011.04.029
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Copper(I) species can be acquired from the reduction of copper(II) by ascorbic acid (AA) in situ, and which in turn quantitative catalyze the azides and alkynes cycloaddition reaction. In this study, propargyl-functionalized ferrocene (propargyl-functionalized Fc) has been modified on the electrode through reacting with azide terminal modified Au electrode via copper(I) catalyzed azides and alkynes cycloaddition (CuAAC) reaction. The electrochemical impedance spectroscopy (EIS) measurement has been applied to test the electron transfer resistance of the Au electrode before and after click reaction. The changes of the fractional surface coverage (theta) with different AA concentrations are characterized. It is found that the theta value has a linear response to the logarithm of AA concentration in the range of 5.0 pmol/L to 1.0 nmol/L with the detection limits of 2.6 pmol/L The sensor shows a good stability and selectivity. And it has been successfully applied to the AA detection in the real samples (urine) with satisfactory results. (C) 2011 Elsevier B.V. All rights reserved.
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收藏
页码：4326 / 4330
页数：5
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