Discovering Human RNA Aptamers by Structure-Based Bioinformatics and Genome-Based In Vitro Selection

被引：0

作者：

Ho, Bao ^{[1
,2
,3
]}

Polanco, Julio ^{[1
,2
,3
]}

Jimenez, Randi ^{[1
,2
,3
]}

Luptak, Andrej ^{[1
,2
,3
]}

机构：

[1] Univ Calif Irvine, Dept Pharmaceut Sci, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92717 USA

[3] Univ Calif Irvine, Dept Chem, Irvine, CA 92717 USA

来源：

RIBOSWITCH DISCOVERY, STRUCTURE AND FUNCTION | 2014年 / 549卷

基金：

美国国家科学基金会;

关键词：

SELEX; LIBRARIES; BINDING; LIGANDS; ATP; EVOLUTION; REVEALS; DNA;

D O I：

10.1016/B978-0-12-801122-5.00002-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro selection and structure-based searches have emerged as useful techniques for the discoveries of structurally complex RNAs with high affinity and specificity toward metabolites. Here, we focus on the design of a human genomic library that serves as the DNA template for in vitro selection of RNA aptamers. In addition, the structural solutions obtained from the in vitro selection can be used for structure-based searches for discovery of analogous aptamers in various genomic databases.

引用

页码：29 / 46

页数：18

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