Latent pH-responsive ratiometric fluorescent cluster based on self-assembled photoactivated SNARF derivatives

被引:10
作者
Nakata, Eiji [1 ]
Yukimachi, Yoshihiro [2 ]
Uto, Yoshihiro [2 ]
Hori, Hitoshi [2 ]
Morii, Takashi [1 ]
机构
[1] Kyoto Univ, Inst Adv Energy, Uji, Kyoto, Japan
[2] Univ Tokushima, Inst Sci & Technol, Grad Sch, Dept Life Syst, Tokushima, Japan
关键词
Self-assembly; fluorescent material; ratiometry; pH indicator; photoactivation; DESIGN; PROBES; MECHANISMS; BIOLOGY; DYES;
D O I
10.1080/14686996.2016.1204888
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
We have developed a self-assembled fluorescent cluster comprising a seminaphthorhodafluor (SNARF) derivative protected by a photoremovable o-nitrobenzyl group. Prior to UV irradiation, a colorless and nonfluorescent cluster was spontaneously assembled in aqueous solution. After UV irradiation, the self-assembled cluster remained intact and showed a large enhancement in pH-responsive fluorescence. The unique pH responsive fluorescent cluster could be used as a dual-emissive ratiometric fluorescent pH probe not only in the test tube but also in HeLa cell cultures. [GRAPHICS]
引用
收藏
页码:431 / 436
页数:6
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