Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads

被引：12

作者：

Sakata, Masayo ^{[1
]}

Yoshimura, Kana ^{[1
]}

Sakamoto, Itsumi ^{[1
]}

Todokoro, Masami ^{[2
]}

Kunitake, Masashi ^{[1
]}

机构：

[1] Kumamoto Univ, Dept Appl Chem & Biochem, Grad Sch Sci & Technol, Kumamoto 8608555, Japan

[2] Chisso Co Ltd, Chiyoda Ku, Tokyo 1008105, Japan

来源：

ANALYTICAL SCIENCES | 2011年 / 27卷 / 02期

关键词：

LIPOPOLYSACCHARIDES; BACTERIAL;

D O I：

10.2116/analsci.27.213

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the beta- and gamma-CyD polymer beads was compared with that of common cationic LPS adsorbents. The gamma-CyD polymer beads selectively removed LPS from a DNA solution (50 mu g mL(-1), pH 6, ionic strength mu = 0.2) containing natural LPS (15 EU mL(-1)), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and < 1%, respectively.

引用

页码：213 / 216

页数：4

共 14 条

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[2]

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[3]

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