Effect of ovulation synchronization program and season on pregnancy to timed artificial insemination in suckled beef cows

This study was conducted to (i) evaluate the requirement for the administration of GnRH coincident with insertion of a progesterone-releasing intravaginal device (PRID) and (ii) the effect of supplementing with 400 IU eCG at PRID removal on pregnancy per AI (P/AI) in spring and autumn calving suckled beef cows, subjected to a 7-d CO-Synch thorn PRID timed artificial insemination (TAI) program. Suckled beef cows (n = 1408) on 62 commercial farms were enrolled and randomly assigned to either of three treatments: 1) cows received a PRID and 100 mu g GnRH on Day 10, followed by 25 mg PGF(2 alpha) at PRID removal (Day -3) and 100 mu g GnRH 72 h later (Day 0) at TAI (Treatment 1; n: spring = 236, autumn = 248); 2) as Treatment 1, but without GnRH at PRID insertion on Day 10 (Treatment 2; n: spring = 232, autumn = 227); 3) as Treatment 1, but cows also received 400 IU eCG at PRID removal on Day -3 (Treatment 3; n: spring = 233, autumn = 232). At Day -10, ovaries were examined by ultrasonography to evaluate the presence or absence of a corpus luteum (CL) and follicle(s) >= 10 mm in diameter. Body condition score (BCS) was assessed on a scale of 1-5. Pregnancy diagnosis was carried out 30-35 d after TAI by transrectal ultrasonography. Data were analyzed using the GENMOD and LOGISTIC procedures of SAS. There was a treatment by season interaction for P/AI (P < 0.001). In spring, overall P/AI was 59.1% (414/701) and was affected by treatment (59.3 v 49.6 v 68.2%, for Treatments 1, 2 and 3, respectively P < 0.05). In contrast, in autumn, overall P/AI (51.5%, 364/707) was unaffected (P > 0.05) by treatment (50.1 v 53.7 v 48.7% for Treatments 1, 2 and 3, respectively). Overall, eCG had a positive effect on P/AI for cows lacking a CL at treatment initiation (P < 0.05). In addition, in cows with low BCS (<= 2.25), eCG supplementation tended (P = 0.09) to improve P/AI. Seasonal differences in response to synchronization treatment may be reflective of different management regimens (grazing v confinement) and breed type and remain to be elucidated. (C) 2021 Elsevier Inc. All rights reserved.