Using forward genetics in Nicotiana benthamiana to uncover the immune signaling pathway mediating recognition of the Xanthomonas perforans effector XopJ4

被引:71
作者
Schultink, Alex [1 ]
Qi, Tiancong [1 ]
Bally, Julia [2 ]
Staskawicz, Brian [1 ]
机构
[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[2] Queensland Univ Technol, Ctr Trop Crops & Biocommod, Brisbane, Qld 4001, Australia
基金
美国食品与农业研究所;
关键词
JIM2; Nicotiana benthamiana; nonhost resistance; plant immunity; plant pathogen; Xanthomonas perforans; XopJ4; ZAR1; CAMPESTRIS PV VESICATORIA; DISEASE RESISTANCE; TOMATO; PROTEINS; PEPPER; PLANTS; GENES; PATHOGENICITY; PSEUDOKINASE; ACTIVATION;
D O I
10.1111/nph.15411
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The immune pathway responsible for perception of the Xanthomonas perforans effector XopJ4 was identified in the plant Nicotiana benthamiana. This pathogen causes significant yield loss in commercial tomato cultivation. Genetic mapping and viral-induced gene silencing were used to identify immune signaling components of the XopJ4 perception pathway in N. benthamiana. Transient complementation assays were performed to determine the functionality of gene variants and co-immunoprecipitation assays were used to gain insight into the molecular mechanism of the pathway. Two N. benthamiana ethyl methanesulfonate (EMS) mutants deficient for XopJ4 perception were identified as having loss-of-function mutations in the gene encoding the nucleotide binding, leucine-rich repeat (NLR) protein NbZAR1. Silencing of a receptor-like cytoplasmic kinase family XII gene, subsequently named XOPJ4 IMMUNITY 2 (JIM2), blocks perception of XopJ4. This study demonstrates the feasibility of conducting mutant screens in N. benthamiana to investigate the genetic basis of the plant immune system and other processes. The identification of NbZAR1 and JIM2 as mediating XopJ4 perception in N. benthamiana supports the model of ZAR1 being involved in the perception of many different pathogen effector proteins with specificity dictated by associated receptor-like cytoplasmic kinases.
引用
收藏
页码:1001 / 1009
页数:9
相关论文
共 47 条
[11]   Type III effector proteins from the plant pathogen Xanthomonas and their role in the interaction with the host plant [J].
Gürlebeck, D ;
Thieme, F ;
Bonas, U .
JOURNAL OF PLANT PHYSIOLOGY, 2006, 163 (03) :233-255
[12]   Exploiting Combinatorial Interactions to Expand NLR Specificity [J].
Innes, Roger W. .
CELL HOST & MICROBE, 2015, 18 (03) :265-267
[13]   Intracellular innate immune surveillance devices in plants and animals [J].
Jones, Jonathan D. G. ;
Vance, Russell E. ;
Dangl, Jeffery L. .
SCIENCE, 2016, 354 (6316)
[14]   Of guards, decoys, baits and traps: pathogen perception in plants by type III effector sensors [J].
Khan, Madiha ;
Subramaniam, Rajagopal ;
Desveaux, Darrell .
CURRENT OPINION IN MICROBIOLOGY, 2016, 29 :49-55
[15]   Bacterial wilt disease: Host resistance and pathogen virulence mechanisms [J].
Kim, Bong-Suk ;
French, Elizabeth ;
Caldwell, Denise ;
Harrington, Emily J. ;
Iyer-Pascuzzi, Anjali S. .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2016, 95 :37-43
[16]   Pepper Suppressor of the G2 Allele of skp1 Interacts with the Receptor-Like Cytoplasmic Kinase1 and Type III Effector AvrBsT and Promotes the Hypersensitive Cell Death Response in a Phosphorylation-Dependent Manner [J].
Kim, Nak Hyun ;
Kim, Dae Sung ;
Chung, Eui Hwan ;
Hwang, Byung Kook .
PLANT PHYSIOLOGY, 2014, 165 (01) :76-91
[17]   Activation of an Arabidopsis Resistance Protein Is Specified by the in Planta Association of Its Leucine-Rich Repeat Domain with the Cognate Oomycete Effector [J].
Krasileva, Ksenia V. ;
Dahlbeck, Douglas ;
Staskawicz, Brian J. .
PLANT CELL, 2010, 22 (07) :2444-2458
[18]   The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a [J].
Lewis, Jennifer D. ;
Lee, Amy Huei-Yi ;
Hassan, Jana A. ;
Wan, Janet ;
Hurley, Brenden ;
Jhingree, Jacquelyn R. ;
Wang, Pauline W. ;
Lo, Timothy ;
Youn, Ji-Young ;
Guttman, David S. ;
Desveaux, Darrell .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (46) :18722-18727
[19]  
Lewis JD, 2011, MOL PLANT PATHOL, V12, P928, DOI [10.1111/J.1364-3703.2011.00719.X, 10.1111/j.1364-3703.2011.00719.x]
[20]  
Lewis JenniferD., 2010, PLOS GENET, V6, P1, DOI DOI 10.1371/J0URNAL.PGEN.1000894