A catalogue of human saliva proteins identified by free flow electrophoresis-based peptide separation and tandem mass spectrometry

被引:132
作者
Xie, HW
Rhodus, NL
Griffin, RJ
Carlis, JV
Griffin, TJ
机构
[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Oral Med Diag & Radiol, Sch Dent, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Therapeut Radiol Radiat Oncol, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Dept Comp Sci & Engn, Minneapolis, MN 55455 USA
关键词
D O I
10.1074/mcp.D500008-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Human saliva has great potential for clinical disease diagnostics. Constructing a comprehensive catalogue of saliva proteins using proteomic approaches is a necessary first step to identifying potential protein biomarkers of disease. However, because of the challenge presented in cataloguing saliva proteins with widely varying abundance, new proteomic approaches are needed. To this end, we used a newly developed approach coupling peptide separation using free flow electrophoresis with linear ion trap tandem mass spectrometry to identify proteins in whole human saliva. We identified 437 proteins with high confidence (false positive rate below 1%), producing the largest catalogue of proteins from a single saliva sample to date and providing new information on the composition and potential diagnostic utility of this fluid. The statistically validated, transparently presented, and annotated dataset provides a model for presenting large scale proteomic data of this type, which should facilitate better dissemination and easier comparisons of proteomic datasets from future studies in saliva.
引用
收藏
页码:1826 / 1830
页数:5
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