Nitrosulfonate S as the substrate for horseradish peroxidase in enzyme-linked immunoassay

被引：0

作者：

Zhu, M ^{[1
]}

Han, SB

Yuan, ZB

Shen, HX

机构：

[1] Univ Sci & Technol China, Grad Sch, Dept Chem, Beijing 100039, Peoples R China

[2] Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 27卷 / 04期

关键词：

nitrosulfonate S; horseradish peroxidase; enzyme-linked immunoassay; spectrophotometry; voltammetry;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new substrate, nitrosulfonate S, for horseradish peroxidase HRP was reported, and the mechanism of oxidative reaction catalyzed by HRP in the presence of H2O2 was discussed primarily. Some kinetic constants of enzyme catalytic reaction were determined, with K-m and K-s of 4.51 x 10(-5) mol/L and 28.1 mol/L.s(-1), respectively. The hydrogen donor was employed in the enzyme-linked spectrophotometric immunoassay and voltammetric enzyme immunoassay, and the highest dilution in the method were 1:1.6 x 10(5) and 1:4.8 x 10(5), respectively.

引用

页码：440 / 443

页数：4

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