Molecular determinants of ammonia and urea conductance in plant aquaporin homologs

被引：72

作者：

Dynowski, Marek ^{[1
]}

Mayer, Maria ^{[1
]}

Moran, Oscar ^{[2
]}

Ludewig, Uwe ^{[1
]}

机构：

[1] Univ Tubingen, ZMBP, D-72076 Tubingen, Germany

[2] CNR, Ist Biofis, I-16149 Genoa, Italy

来源：

FEBS LETTERS | 2008年 / 582卷 / 16期

关键词：

channel; molecular dynamics; nitrogen; Arabidopsis;

D O I：

10.1016/j.febslet.2008.06.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2; 1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH3, and a residue without direct contact to the pore lumen was critical for conduction in the mutants. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2458 / 2462

页数：5

共 23 条

[1] Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat [J].