Antileukemic Cell Proliferation of Active Compounds from Kaffir Lime (Citrus hystrix) Leaves

被引：21

作者：

Anuchapreeda, Songyot ^{[1
,2
,3
,4
]}

Chueahongthong, Fah ^{[1
]}

Viriyaadhammaa, Natsima ^{[1
]}

Panyajai, Pawaret ^{[1
]}

Anzawa, Riki ^{[2
]}

Tima, Singkome ^{[1
,3
,4
]}

Ampasavate, Chadarat ^{[5
]}

Saiai, Aroonchai ^{[6
]}

Rungrojsakul, Methee ^{[7
]}

Usuki, Toyonobu ^{[2
]}

Okonogi, Siriporn ^{[4
,5
]}

机构：

[1] Chiang Mai Univ, Fac Associated Med Sci, Dept Med Technol, Chiang Mai 50200, Thailand

[2] Sophia Univ, Fac Sci & Technol, Dept Mat & Life Sci, Tokyo 1028554, Japan

[3] Chiang Mai Univ, Fac Associated Med Sci, Canc Res Unit, Associated Med Sci AMS CRU, Chiang Mai 50200, Thailand

[4] Chiang Mai Univ, Res Ctr Pharmaceut Nanotechnol, Chiang Mai 50200, Thailand

[5] Chiang Mai Univ, Dept Pharmaceut Sci, Fac Pharm, Chiang Mai 50200, Thailand

[6] Chiang Mai Univ, Dept Chem, Fac Sci, Chiang Mai 50200, Thailand

[7] Chandrakasem Rajabhat Univ, Coll Alternat Med, Bangkok 10900, Thailand

来源：

MOLECULES | 2020年 / 25卷 / 06期

关键词：

kaffir lime; phytol; lupeol; Wilms' tumor 1; leukemia; antiproliferation; WT1; GENE-EXPRESSION; PURE CURCUMIN; EXTRACTS; PLANTS; PHYTOL; LEAF; OILS;

D O I：

10.3390/molecules25061300

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms' tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation.

引用

页数：16

共 33 条

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