Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

被引:24
作者
Sartika, Dewi [1 ]
Wang, Chih-Hsin [2 ]
Wang, Ding-Han [3 ]
Cherng, Juin-Hong [4 ,5 ]
Chang, Shu-Jen [1 ,6 ]
Fan, Gang-Yi [1 ,7 ]
Wang, Yi-Wen [4 ]
Lee, Chian-Her [8 ]
Hong, Po-Da [9 ]
Wang, Chih-Chien [10 ]
机构
[1] Natl Def Med Ctr, Lab Adult Stem Cell & Tissue Regenerat, Taipei 114, Taiwan
[2] Triserv Gen Hosp, Dept Plast & Reconstruct Surg, Natl Def Med Ctr, Taipei 114, Taiwan
[3] Natl Yang Ming Univ, Sch Dent, Dept Dent, Taipei 112, Taiwan
[4] Natl Def Med Ctr, Dept & Grad Inst Biol & Anat, Taipei 114, Taiwan
[5] Natl Taipei Univ Nursing & Hlth Sci, Dept Gerontol Hlth Care, Taipei 112, Taiwan
[6] Triserv Gen Hosp, Div Rheumatol Immunol Allergy, Dept Internal Med, Natl Def Med Ctr, Taipei 114, Taiwan
[7] Triserv Gen Hosp, Div Urol, Dept Surg, Natl Def Med Ctr, Taipei 114, Taiwan
[8] Taipei Med Univ, Sch Med, Dept Orthoped, Taipei Med Univ Hosp,Coll Med, Taipei 110, Taiwan
[9] Natl Taiwan Univ Sci & Technol, Dept Mat Sci & Engn, Taipei 106, Taiwan
[10] Triserv Gen Hosp, Dept Orthoped Surg, Natl Def Med Ctr, Taipei 114, Taiwan
关键词
silk fibroin; scaffold; adipose stem cells; bone regeneration; calvarial defects; bone tissue engineering; IN-VIVO; BIOMATERIALS; REPAIR; BMP-2; DIFFERENTIATION; INFLAMMATION; MEMBRANES; FRACTURE;
D O I
10.3390/polym12040853
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications.
引用
收藏
页数:15
相关论文
共 50 条
[1]  
Amini Ami R., 2012, Critical Reviews in Biomedical Engineering, V40, P363
[2]   The role of mesenchymal stem cells in maintenance and repair of bone [J].
Bielby, Robert ;
Jones, Elena ;
McGonagle, Dennis .
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, 2007, 38 :S26-S32
[3]  
Blair HC, 2017, TISSUE ENG PART B-RE, V23, P268, DOI [10.1089/ten.teb.2016.0454, 10.1089/ten.TEB.2016.0454]
[4]   Recent advances in bone tissue engineering scaffolds [J].
Bose, Susmita ;
Roy, Mangal ;
Bandyopadhyay, Amit .
TRENDS IN BIOTECHNOLOGY, 2012, 30 (10) :546-554
[5]   A multi-functional scaffold for tissue regeneration: The need to engineer a tissue analogue [J].
Causa, Filippo ;
Netti, Paolo A. ;
Ambrosio, Luigi .
BIOMATERIALS, 2007, 28 (34) :5093-5099
[6]   Fracture healing under healthy and inflammatory conditions [J].
Claes, Lutz ;
Recknagel, Stefan ;
Ignatius, Anita .
NATURE REVIEWS RHEUMATOLOGY, 2012, 8 (03) :133-143
[7]   Testing the Critical Size in Calvarial Bone Defects: Revisiting the Concept of a Critical-Size Defect [J].
Cooper, Gregory M. ;
Mooney, Mark P. ;
Gosain, Arun K. ;
Campbell, Phil G. ;
Losee, Joseph E. ;
Huard, Johnny .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2010, 125 (06) :1685-1692
[8]   Blood Vessel Formation and Bone Regeneration Potential of the Stromal Vascular Fraction Seeded on a Calcium Phosphate Scaffold in the Human Maxillary Sinus Floor Elevation Model [J].
Farre-Guasch, Elisabet ;
Bravenboer, Nathalie ;
Helder, Marco N. ;
Schulten, Engelbert A. J. M. ;
ten Bruggenkate, Christiaan M. ;
Klein-Nulend, Jenneke .
MATERIALS, 2018, 11 (01)
[9]   Current Concepts of Bone Tissue Engineering for Craniofacial Bone Defect Repair [J].
Fishero, Brian Alan ;
Kohli, Nikita ;
Das, Anusuya ;
Christophel, John Jared ;
Cui, Quanjun .
CRANIOMAXILLOFACIAL TRAUMA & RECONSTRUCTION, 2015, 8 (01) :23-30
[10]   Buried alive: How osteoblasts become osteocytes [J].
Franz-Odendaal, TA ;
Hall, BK ;
Witten, PE .
DEVELOPMENTAL DYNAMICS, 2006, 235 (01) :176-190