Resin-supported labeling reagents

被引:26
作者
Adamczyk, M [1 ]
Fishpaugh, JR [1 ]
Mattingly, PG [1 ]
机构
[1] Abbott Labs, Dept Chem, Div Diagnost, Abbott Pk, IL 60064 USA
关键词
D O I
10.1016/S0960-894X(98)00711-2
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Resin-supported fluorescein, coumarin, acridinium, and biotin active esters were prepared from a new N-hydroxysuccinimidyl resin in high yield. The active esters were used to prepare representative conjugates with estriol, thyroxine, phenytoin, and desipramine haptens without need for purification beyond removal of the spent resin. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:217 / 220
页数:4
相关论文
共 13 条
[1]   Letter: Detection of reaction intermediates by flow injection electrospray ionization mass spectrometry: reaction of chemiluminescent N-sulfonylacridinium-9-carboxamides with hydrogen peroxide [J].
Adamczyk, M ;
Fishpaugh, JR ;
Gebler, JC ;
Mattingly, PG ;
Shreder, K .
EUROPEAN MASS SPECTROMETRY, 1998, 4 (02) :121-125
[2]   Preparation of succinimidyl and pentafluorophenyl active esters of 5- and 6-carboxyfluorescein [J].
Adamczyk, M ;
Fishpaugh, JR ;
Heuser, KJ .
BIOCONJUGATE CHEMISTRY, 1997, 8 (02) :253-255
[3]   AN EASY PREPARATION OF HAPTEN ACTIVE ESTERS VIA SOLID SUPPORTED EDAC [J].
ADAMCZYK, M ;
FISHPAUGH, JR ;
MATTINGLY, PG .
TETRAHEDRON LETTERS, 1995, 36 (46) :8345-8346
[4]   Immunoassay reagents for thyroid testing.: 3.: Determination of the solution binding affinities of a T4 monoclonal antibody Fab fragment for a library of thyroxine analogs using surface plasmon resonance [J].
Adamczyk, M ;
Johnson, DD ;
Mattingly, PG ;
Moore, JA ;
Pan, Y .
BIOCONJUGATE CHEMISTRY, 1998, 9 (01) :23-32
[5]   Estradiol-mimetic probes.: Preparation of 17α-(6-aminohexynyl)estradiol biotin, fluorescein and acridinium conjugates [J].
Adamczyk, M ;
Chen, YY ;
Moore, JA ;
Mattingly, PG .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1998, 8 (11) :1281-1284
[6]   Neopentyl 3-triflyloxypropanesulfonate. A reactive sulfopropylation reagent for the preparation of chemiluminescent labels [J].
Adamczyk, M ;
Chen, YY ;
Mattingly, PG ;
Pan, Y ;
Rege, S .
JOURNAL OF ORGANIC CHEMISTRY, 1998, 63 (16) :5636-5639
[7]   Synthesis of 6β-aminoestradiol and its biotin, acridinium, and fluorescein conjugates [J].
Adamczyk, M ;
Mattingly, PG ;
Reddy, RE .
STEROIDS, 1998, 63 (03) :130-134
[8]  
ADAMCZYK M, IN PRESS TETRAHEDRON
[9]   LONG-WAVELENGTH CHLORIDE-SENSITIVE FLUORESCENT INDICATORS [J].
BIWERSI, J ;
TULK, B ;
VERKMAN, AS .
ANALYTICAL BIOCHEMISTRY, 1994, 219 (01) :139-143
[10]  
FLENTGE CA, Patent No. 108403