Comprehensive two-dimensional gas chromatography with capillary flow modulation to separate FAME isomers

被引:30
作者
Manzano, Pilar [1 ]
Arnaiz, Esther [1 ]
Carlos Diego, Juan [1 ]
Toribio, Laura [1 ]
Garcia-Viguera, Cristina [2 ]
Luis Bernal, Jose [1 ]
Bernal, Jose [1 ,3 ]
机构
[1] Univ Valladolid, Analyt Chem Grp, IU CINQUIMA, E-47002 Valladolid, Spain
[2] CEBAS CSIC, Dept Food Sci & Technol, Murcia, Spain
[3] CSIC, Inst Ind Fermentat, E-28006 Madrid, Spain
关键词
GCxGC; SFE; Capillary flow modulation; Orthogonality; FAME; Broccoli; FATTY-ACIDS; BROCCOLI FLORETS; SAMPLES; PROFILE; OIL;
D O I
10.1016/j.chroma.2011.02.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method to separate FAME and the linoleic and linolenic acids isomers by GCxGC using an apparatus equipped with a capillary flow technology (CFT) based modulator and a FID detector has been developed. Four different column combinations (one conventional and three inverted phase sets) were used in these experiments. The conventional set first involved a DB5-MS non-polar column followed by a highly polar HP-INNOWax column in the second dimension. The inverted phase set comprised of a highly polar BPX-70 column in the first dimension and a non-polar ZB5-MS column for the second dimension. Furthermore, the influence of the length of the second dimension column on FAME isomer separation was studied in the inverted phase sets, along with other parameters like the modulation time and column flow. The best results in terms of the time required for the analysis and number of FAME identified with the inverted set were achieved with the shorter second dimension column. After supercritical fluid extraction, the method was applied to identify FAMEs in broccoli leaves from three different cultivars (Naxos, Nubia and Viola). (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:4952 / 4959
页数:8
相关论文
共 46 条
[1]   Twin comprehensive two-dimensional gas chromatographic system: concept and applications [J].
Adahchour, M ;
Jover, E ;
Beens, J ;
Vreuls, RJJ ;
Brinkman, UAT .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1086 (1-2) :128-134
[2]   Using comprehensive two-dimensional gas chromatography for the analysis of oxygenates in middle distillates -: I.: Determination of the nature of biodiesels blend in diesel fuel [J].
Adam, Frederick ;
Bertoncini, Fabrice ;
Coupard, Vincent ;
Charon, Nadege ;
Thiebaut, Didier ;
Espinat, Didier ;
Hennion, Marie-Claire .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1186 (1-2) :236-244
[3]   Supercritical fluid extraction of lipids from broccoli leaves [J].
Arnaiz, Esther ;
Bernal, Jose ;
Teresa Martin, Maria ;
Garcia-Viguera, Cristina ;
Luis Bernal, Jose ;
Toribio, Laura .
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2011, 113 (04) :479-486
[4]   Nutrient contents of kale (Brassica oleraceae L. var. acephala DC.) [J].
Ayaz, FA ;
Glew, RH ;
Millson, M ;
Huang, HS ;
Chuang, LT ;
Sanz, C ;
Hayirlioglu-Ayaz, S .
FOOD CHEMISTRY, 2006, 96 (04) :572-579
[5]   (n-7) and (n-9) cis-monounsaturated fatty acid contents of 12 Brassica species [J].
Barthet, Veronique J. .
PHYTOCHEMISTRY, 2008, 69 (02) :411-417
[6]   Polyunsaturated fatty acids: Biochemical, nutritional and epigenetic properties [J].
Benatti, P ;
Peluso, G ;
Nicolai, R ;
Calvani, M .
JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 2004, 23 (04) :281-302
[7]   Use of supercritical fluid extraction and gas chromatography-mass spectrometry to obtain amino acid profiles from several genetically modified varieties of maize and soybean [J].
Bernal, J. L. ;
Nozal, M. J. ;
Toribio, L. ;
Diego, C. ;
Mayo, R. ;
Maestre, R. .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1192 (02) :266-272
[8]   Creating a fatty acid methyl ester database for lipid profiling in a single drop of human blood using high resolution capillary gas chromatography and mass spectrometry [J].
Bicalho, Beatriz ;
David, Frank ;
Rumplel, Klaus ;
Kindt, Erick ;
Sandra, Pat .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1211 (1-2) :120-128
[9]  
Cheng F., 2008, J CHROMATOGR A, V1188, P274
[10]   Comprehensive two dimensional gas chromatography review [J].
Cortes, Hernan J. ;
Winniford, Bill ;
Luong, Jim ;
Pursch, Matthias .
JOURNAL OF SEPARATION SCIENCE, 2009, 32 (5-6) :883-904