Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

被引：22

作者：

Zhang Jun ^{[1
]}

Zhang Jing ^{[2
]}

Li MengJie ^{[2
]}

Wu YouJun ^{[2
]}

Fan YingYing ^{[1
]}

Zhou Yang ^{[1
]}

Tan LiLi ^{[2
]}

Shao ZhiHua ^{[1
]}

Shi HongJun ^{[1
]}

机构：

[1] Tongji Univ, Sch Med, Dept Regenerat Med, Shanghai 200092, Peoples R China

[2] Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2011年 / 24卷 / 02期

基金：

上海市自然科学基金;

关键词：

Nickel; DNA methylation; RAR-beta; 2; RASSF1A; CDKN2A; Quantitative Real-time Methylation Specific PCR; ACID RECEPTOR-BETA; DNA METHYLATION; PROMOTER METHYLATION; BREAST-CANCER; RAR-BETA; HYPERMETHYLATION; EXPRESSION; PCR;

D O I：

10.3967/0895-3988.2011.02.011

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Objective To investigate the expression variation of RAR-beta 2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni3S2) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-beta 2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.

引用

页码：163 / 171

页数：9