Multicolor Imaging of cell surface proteins

被引：61
作者：
Vivero-Pol, L
George, N
Krumm, H
Johnsson, K [1 ]
Johnsson, N
机构：
[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland
[2] Univ Munster, Inst Med Biochem, Ctr Mol Biol Inflammat, D-48149 Munster, Germany
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 37期
关键词：
D O I：
10.1021/ja0533850
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report on a method for the multicolor imaging of cell surface proteins which is based on the labeling of carrier protein (CP) fusion proteins with different fluorophores. In one application, different generations of a cell surface protein can be sequentially labeled to discriminate between old and newly made copies. In another application, fusions to different CPs can be selectively labeled with different fluorophores in one sample. Both applications open up new ways for studying the properties of cell surface proteins of living cells. Copyright © 2005 American Chemical Society.
引用
收藏
页码：12770 / 12771
页数：2
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