Overexpression of IbMPK3 increases low-temperature tolerance in transgenic sweetpotato

被引：6

作者：

Jin, Rong ^{[1
]}

Kim, Ho Soo ^{[2
]}

Yu, Tao ^{[3
]}

Liu, Ming ^{[1
]}

Yu, Wenhui ^{[4
,5
]}

Zhao, Peng ^{[1
]}

Zhang, Aijun ^{[1
]}

Zhang, Qiangqiang ^{[1
]}

Liu, Yaju ^{[1
]}

Cao, Qinghe ^{[1
]}

Kwak, Sang-Soo ^{[2
]}

Tang, Zhonghou ^{[1
]}

机构：

[1] Minist Agr, Xuzhou Sweetpotato Res Ctr, China Key Lab Sweetpotato Biol & Genet Breeding, Xuzhou Inst Agr Sci Jiangsu, Xuzhou, Jiangsu, Peoples R China

[2] Korea Res Inst Biosci & Biotechnol KRIBB, Plant Syst Engn Res Ctr, 125 Gwakak Ro, Daejeon 34141, South Korea

[3] Liaoning Acad Agr Sci, Shenyang, Peoples R China

[4] Jiangsu Normal Univ, Sch Life Sci, Xuzhou, Jiangsu, Peoples R China

[5] Quzhou Acad Agr & Forestry Sci, Quzhou, Peoples R China

来源：

PLANT BIOTECHNOLOGY REPORTS | 2022年 / 16卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Low-temperature stress; Overexpression; IbMPK3; Sweetpotato; PROTEIN-KINASE KINASE; MAIZE ZEA-MAYS; STRESS TOLERANCE; SIGNALING PATHWAY; OXIDATIVE STRESS; SUPEROXIDE-DISMUTASE; FUNCTIONAL-ANALYSIS; FREEZING TOLERANCE; MAPK CASCADES; COLD;

D O I：

10.1007/s11816-021-00730-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sweetpotato is an important crop that is very sensitive to low temperatures. Mitogen-activated protein kinase (MAPK) is involved in plant growth and development and is responsive to many environmental stresses. IbMPK3 is strongly regulated by low-temperature stress. We studied the function of IbMPK3 and how it may enhance the adaptability of sweetpotato plants to low-temperature stress. Transgenic sweetpotato plants overexpressing IbMPK3 were generated, and three transgenic lines with the highest expression level of IbMPK3 were used for low-temperature tests. Transgenic plants exposed to low-temperature stress had less damage associated with higher photosynthesis efficiency and less cell membrane damage. IbMPK3-overexpressing transgenic plants could modulate reactive oxygen species (ROS) metabolism with lower levels of O-2(-) and H2O2 accumulation and higher enzymatic activities than WT plants. Transcript expression levels of some ROS-related genes (IbCAT, IbCu-ZnSOD, and IbCAT) and stress-responsive genes (IbP3B and IbCOR27) were significantly upregulated in transgenic plants compared to WT. These results indicate that IbMPK3 has an important role in sweetpotato response to low temperatures.

引用

页码：91 / 100

页数：10

共 53 条

[11] Elevated compartmentalization of Na+ into vacuoles improves salt and cold stress tolerance in sweet potato (Ipomoea batatas) [J].