Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering

被引：96
作者：
Hirata, Eri [1 ]
Uo, Motohiro [2 ]
Takita, Hiroko [3 ]
Akasaka, Tsukasa [2 ]
Watari, Fumio [2 ]
Yokoyama, Atsuro [1 ]
机构：
[1] Hokkaido Univ, Grad Sch Dent Med, Div Oral Funct Sci, Dept Oral Funct Prosthodont,Kita Ku, Sapporo, Hokkaido 0608586, Japan
[2] Hokkaido Univ, Grad Sch Dent Med, Dept Biomed Dent Mat & Sci, Kita Ku, Sapporo, Hokkaido 0608586, Japan
[3] Hokkaido Univ, Grad Sch Dent Med, Support Sect Educ & Res, Kita Ku, Sapporo, Hokkaido 0608586, Japan
来源：
CARBON | 2011年 / 49卷 / 10期
基金：
日本学术振兴会;
关键词：
SUBCUTANEOUS TISSUE; OSTEOBLAST PHENOTYPE; IN-VIVO; EXTRACELLULAR-MATRIX; APATITE FORMATION; BIOCOMPATIBILITY; DIFFERENTIATION; RATS; EXPRESSION; COMPOSITE;
D O I：
10.1016/j.carbon.2011.04.002
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Surface coating treatment with multiwalled carbon nanotubes (MWCNTs) was applied to 3D collagen scaffold for bone tissue engineering. Rat primary osteoblasts (ROBs) were cultured on an MWCNT-coated collagen sponge (MWCNT-coated sponge) in a 3D dynamic flow cell culture system and differentiation markers were measured. Alkaline phosphatase activity at 1 day, and calcium and osteopontin contents of the MWCNT-coated sponges at 7 days were significantly higher than those of uncoated sponges. ROBs on the MWCNT-coated sponge differentiated earlier than on the uncoated sponge. In addition, the tissue response to the MWCNT-coated sponge was evaluated. Slight inflammation was observed around MWCNTs at 7 and 28 days after implantation in subcutaneous tissue. Significantly more bone formation was observed around the MWCNT-coated sponges than around the uncoated sponges and new bone attached to MWCNTs directly at 28 and 56 days after implantation in the femur. Moreover, at 28 days after implantation of the MWCNT-coated sponge with osteoblasts cultured for 1 day, bone tissues were successfully formed in the pores according to its honeycomb structure. Therefore, MWCNT coating appears to be effective for bone tissue engineering. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3284 / 3291
页数：8
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