Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization

Rac is a small GTPase of the Rho family that mediates stimulus-induced actin cytoskeletal reorganization to generate lamellipodia(1-5). Little is known about the signalling pathways that link pac activation to changes in actin filament dynamics. Cofilin is known to be a potent regulator of actin filament dynamics(6-10), and its ability to bind and depolymerize actin is abolished by phosphorylation of serine residue at 3 (refs 11, 12); however, the kinases responsible for this phosphorylation have not been identified. Here we show that LIM-kinase 1 (LIMK-1), a serine/threonine kinase containing LIM and PDZ domains(13-16), phosphorylates cofilin at Ser3, both in vitro and in vivo. When expressed in cultured cells, LIMK-1 induces actin reorganization and reverses cofilin-induced actin depolymerization. Expression of an inactive form of LIMK-1 suppresses lamellipodium formation induced by pac or insulin. Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in,Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin.